黄瓜核心种质白粉病抗性的全基因组关联分析  

Genome-Wide Association Study of Powdery Mildew Resistance of Cucumber Core Germplasms

作　　者：贾会霞 李锡香[1] 宋江萍[1] 林毓娥[2] 张晓辉[1] 邱杨[1] 阳文龙 娄群峰[3] 王海平[1] JIA Huixia;LI Xixiang;SONG Jiangping;LIN Yu’e;ZHANG Xiaohui;QIU Yang;YANG Wenlong;LOU Qunfeng;WANG Haiping(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and Germplasm Creation in the Ministry of Agriculture,Beijing 100081,China;Guangdong Key Laboratory for New Technology Research of Vegetables,Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京100081 [2]广东省农业科学院蔬菜研究所,广东省蔬菜新技术研究重点实验室,广州510640 [3]南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,南京210095

出　　处：《园艺学报》2021年第7期1371-1385,共15页Acta Horticulturae Sinica

基　　金：国家“十三五”重点研发计划项目(2016YFD0100204-1);中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS);中央级公益性科研院所基本科研业务费专项(IVF-BRF2021001);农业农村部作物种质资源保护项目;农业农村部作物种质资源安全保存项目;国家园艺种质北京蔬菜分库运行服务项目(NHGRC2021-NH0);国家作物种质资源数据中心观测监测任务项目(ZX01NK-03)。

摘　　要：以231份来自世界各地的黄瓜核心种质为材料,于2017和2018年在北京和广州进行白粉病抗性鉴定,利用基因组重测序检测的950382个SNPs进行全基因组关联分析。结果表明,黄瓜白粉病在广州地区的自然发病率显著高于北京地区,不同种质在两年病情指数(DI)之间存在较高的相关性;基于混合线性模型,共检测到12个显著关联信号,分布于1号、5号和6号染色体上,其中,pm1.1在北京的两年中被重复检测到,pm5.7在广州的两年中被重复检测到;显著关联信号中共有68个超过阈值[–log 10(P value)=6.0]的SNP,包含1个外显子区非同义突变SNP(Chr5:16676542),位于编码异嗜性和多嗜性逆转录病毒受体蛋白基因XPR(Csa5G471600)上;在与白粉病抗性相关联SNP的LD区段内共检测到63个候选基因,其中7个候选基因的表达量在高抗材料接种白粉病前后出现显著差异。In this study,231 cucumber core germplasms collected from the world were used for resistance identification of powdery mildew in Beijing and Guangzhou in 2017 and 2018,respectively.A total of 950382 SNPs detected by genome re-sequencing were used for genome-wide association analysis.The results showed that the natural incidence of cucumber powdery mildew in Guangzhou was significantly higher than that in Beijing.High correlation was detected between the two-year disease index among different germplasms.Based on the mixed linear model,12 significantly associated signals were detected,and they distributed on chromosomes 1,5 and 6.Among them,pm1.1 was detected repeatedly in two-year in Beijing,while pm5.7 was detected repeatedly in two-year in Guangzhou.These significantly associated signals contained 68 SNPs that exceeded the threshold of–log 10(P value)with 6.0.Among them,a non-synonymous mutant SNP(Chr5:16676542)located on the exon of xenotropic and polytropic retrovirus receptor(Csa5G471600).A total of 63 candidate genes were found in the LD block of SNPs associated with powdery mildew resistance,among which seven candidate genes showed significant differential expression after being infected with powdery mildew in the cucumber material with high resistance.

关 键 词：黄瓜 白粉病 全基因组关联分析 单核苷酸多态性 候选基因 

分 类 号：S642.2[农业科学—蔬菜学]