基于RNA-seq的谷子萌芽期抗旱相关基因挖掘与分析  

Identification and Analysis of Drought Related Genes in Foxtail Millet (Setaria italica L.) at Germination Stage Based on Transcriptome Sequencing

作　　者：代小冬[1] 朱灿灿[1] 宋迎辉[1] 王春义[1] 代书桃[1] 秦娜[1] 李君霞[1] DAI Xiaodong;ZHU Cancan;SONG Yinghui;WANG Chunyi;DAI Shutao;QIN Na;LI Junxia(Cereal Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou,Henan 450002)

机构地区：[1]河南省农业科学院粮食作物研究所,河南郑州450002

出　　处：《核农学报》2021年第8期1761-1770,共10页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(U1504314);国家现代农业产业技术体系专项资金项目(nycytx-CARS-06)。

摘　　要：为了明确响应干旱胁迫的关键基因,解析谷子抗旱机制,本研究以谷子抗旱品种山西2010和干旱敏感品种K359*M4-1为材料,应用RNA-seq技术对两个品种干旱胁迫前后萌发期种子进行转录组测定。结果表明,在山西2010和K359*M4-1中分别鉴定出2 300个和3 652个差异表达基因(DEG),包括编码类锌诱导的促进因子、类萌发素蛋白、蛋白磷酸化酶、转运蛋白、胚胎发育晚期丰富蛋白(LEA)、转录因子、过氧化物酶等基因。通过对鉴定到的DEG进行GO和KEGG代谢途径富集分析,发现山西2010和K359*M4-1中的DEG分别富集在52个和21个生物学过程。KEGG富集分析发现,DEG主要富集在淀粉和蔗糖代谢,植物激素信号转导,光合作用-天线蛋白,苯丙素生物合成,角质、亚氨酸和蜡生物合成,次生代谢物生物合成等途径。本研究结果为挖掘谷子抗旱关键基因、解析谷子抗旱机制奠定了基础。In order to identify the key genes responsive to drought stress and to elucidate the underlying mechanism in drought tolerance of foxtail millet, the germinating seeds of drought tolerant line Shanxi2010 and drought sensitive K359*M4-1 were subjected to RNA-seq before and post drought treatment. The data showed that 2300 and 3652 differentially expressed genes(DEG) were identified in Shanxi 2010 and K359*M4-1 respectively, including genes encoding zinc induced facilitator like protein, germination like protein, protein phosphorylase, transporter protein, late embryogenesis abundant protein(LEA), transcriptional factors, peroxidase and others. The GO(gene ontology) terms and KEGG(Kyoto Encyclopedia of genes and genomes) metabolic pathway enrichment analysis were carried out with the DEGs identified by different comparison groups. DEGs in these two lines were enriched in 52 and 21 biological processes, respectively. KEGG enrichment analysis indicated that DEGs were mainly enriched in the pathways including ‘starch and sucrose metabolism’, ‘plant hormone signal transduction’, ‘photosynthesis antenna protein’, ‘phenylpropanoid biosynthesis’, and ‘cutin, imine and wax biosynthesis’ and ‘secondary metabolite biosynthesis’. This study laid a foundation for excavating key genes related to drought tolerance and demonstrating the genes network that involved in drought responses in foxtail millet.

关 键 词：谷子 萌芽期 抗旱性 RNA-SEQ 

分 类 号：S515[农业科学—作物学]