lncRNA TTN-AS1靶向抑制miR-1271的表达对肺癌细胞增殖、侵袭和凋亡的影响及机制  

The effect of lncRNA TTN-AS1 targeted inhibition of miR-1271 expression on the proliferation,invasion and apoptosis of lung cancer cells and its mechanism

作　　者：陈哲 王勤宁 黄璐捷 史信宝[1] 胡静[2] CHEN Zhe;WANG Qinning;HUANG Lujie;SHI Xinbao;HU Jing(Department of Cardiothoracic Surgery,Ningbo Medical Center Lihuili Hospital,Ningbo 315040;Department of Radiotherapy,Ningbo Medical Center Lihuili Hospital,Ningbo 315040,China)

机构地区：[1]宁波市医疗中心李惠利医院心胸外科,浙江宁波315040 [2]宁波市医疗中心李惠利医院放疗科,浙江宁波315040

出　　处：《温州医科大学学报》2021年第8期632-638,共7页Journal of Wenzhou Medical University

基　　金：浙江省自然科学基金资助项目(LQ16HI60002)。

摘　　要：目的:探讨lncRNA TTN-AS1调控miR-1271表达对肺癌细胞增殖、侵袭和凋亡的影响及机制。方法:在人肺癌A549细胞中转染TTN-AS1 siRNA或miR-1271 mimics,采用qRT-PCR检测TTN-AS1和miR-1271的表达,CCK-8实验检测细胞增殖,Transwell实验检测细胞侵袭,流式细胞术检测细胞凋亡,Western blot检测PI3K、p-AKT、PCNA、E-cadherin和cleaved caspase 3表达。共转染miR-1271 inhibitors和TTN-AS1 siRNA,观察抑制miR-1271对抑制TTN-AS1诱导的A549细胞增殖、侵袭和凋亡的影响。双荧光素酶报告基因实验检测TTN-AS1和miR-1271及miR-1271和PDK1的靶向作用关系,Western blot分析过表达或抑制TTN-AS1/miR-1271对PDK1表达的影响。结果:抑制TTN-AS1及过表达miR-1271均可明显抑制A549细胞增殖和侵袭能力,促进细胞凋亡,下调PI3K、p-AKT和PCNA表达,上调E-cadherin和cleaved caspase 3表达(P<0.05)。抑制miR-1271可逆转抑制TTN-AS1对A549细胞增殖和侵袭能力的抑制作用以及对细胞凋亡的促进作用。TTNAS1和PDK1与miR-1271均存在靶向调控关系。结论:lncRNA TTN-AS1可通过靶向调节miR-1271/PDK1并抑制PI3K/AKT信号通路降低A549细胞增殖和侵袭能力,促进细胞凋亡。Objective:To investigate the effect of lncRNA TTN-AS1 on the proliferation,invasion and apoptosis of lung cancer cells by regulating miR-1271 expression.Methods:Human lung cancer A549 cells were transfected with TTN-AS1 siRNA or miR-1271 mimics.QRT-PCR was used to detect the expression of TTN-AS1 and miR-1271,CCK-8 test to detect cell proliferation,Transwell test to detect cell invasion,flow cytometry to detect cell apoptosis and Western blot to detect the expression of PI3K,p-AKT,PCNA,E-cadherin and cleaved caspase 3.miR-1271 inhibitors and TTN-AS1 siRNA were co-transfected and the effect of miR-1271 inhibition on the proliferation,invasion and apoptosis of A549 cells induced by TTN-AS1 was observed.The dual luciferase reporter gene experiment was performed to detect the targeting effect of TTN-AS1/miR-1271 and miR-1271/PDK1.The effect of over-expression or inhibition of TTN-AS1/miR-1271 on PDK1 expression was analyzed by Western blot.Results:Inhibition of TTN-AS1 and over-expression of miR-1271 could significantly inhibit the proliferation and invasion of A549 cells,promote apoptosis,down-regulate the expression of PI3K,p-AKT and PCNA,and up-regulate the expression of E-cadherin and cleaved caspase 3(P<0.05).Inhibition of miR-1271 could reverse the inhibitory effect of TTN-AS1 on the proliferation,invasion of A549 cells and the promotion of apoptosis as well.Both TTN-AS1 and PDK1 had a targeted regulatory relationship with miR-1271.Conclusion:lncRNA TTN-AS1 can inhibit the proliferation and invasion of A549 cells and promote apoptosis by targeting miR-1271/PDK1 and inhibiting PI3K/AKT signaling pathway.

关 键 词：肺癌 lncRNA TTN-AS1 miR-1271 PI3K/AKT信号通路 侵袭 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]