循环外泌体介导的lncRNA HOXA11-AS通过上调SOX4促进血管内皮细胞增殖  

The value of circulating exosomes-mediated lncRNA HOXA11-AS in the proliferation of vascular endothelial cell by up-regulating SOX4

作　　者：王平[1] 孟立平[1] 刘龙斌 彭放[1] WANG Ping;MENG Liping;LIU Longbin;PENG Fang(Department of Cardiology,Shaoxing People’s Hospital(Shaoxing Hospital of Zhejiang University),Shaoxing 312000,China;Department of Cardiology,Affiliated Hospital of Shaoxing College of Arts and Sciences(Shaoxing Municipal Hospital),Shaoxing 312000,China)

机构地区：[1]绍兴市人民医院(浙江大学绍兴医院)心内科,浙江绍兴312000 [2]绍兴文理学院附属医院(绍兴市立医院)心内科,浙江绍兴312000

出　　处：《温州医科大学学报》2021年第8期639-645,共7页Journal of Wenzhou Medical University

基　　金：浙江省科技厅公益项目(LGF19H020002);国家自然科学基金青年基金资助项目(82000252);浙江省卫生厅项目(2021RC032);绍兴市医卫类科技计划项目(2020A13018)。

摘　　要：目的:探索循环外泌体对内皮细胞增殖能力的影响及其可能机制。方法:收集支架内再狭窄患者(n=199)及对照组患者(n=32)血浆并提取外泌体,用患者血浆外泌体干预经雷帕霉素诱导的内皮细胞增殖抑制模型,MTT法测各组细胞的增殖能力,Western blot检测细胞中PCNA、Ki67的表达情况。基因芯片检测两组外泌体中差异的非编码RNA,qRT-PCR检测lncRNA HOXA11-AS表达情况。结果:与对照组比,雷帕霉素组细胞增殖能力减弱;PCNA、Ki67表达减少(P<0.05);相比于雷帕霉素组,对照组外泌体干预后内皮细胞增殖能力增强;PCNA、Ki67表达增加(P<0.05)。芯片结果显示lncRNA HOXA11-AS在ISR外泌体中表达减少(P<0.05)。pHOXA11-AS可以促进内皮细胞的增殖能力,增加细胞中PCNA、Ki67、SOX4的表达(P<0.05)。结论:血浆外泌体中的lncRNA HOXA11-AS可能是通过上调内皮细胞中SOX4的表达,增加内皮细胞的增殖能力。Objective:To explore the effect of circulating exosomes on the proliferation ability of endothelial cells and its possible mechanism.Methods:The plasma of patients with in-stent restenosis(ISR,n=199)and Non-ISR(n=32)was collected and the exosomes in the plasma were extracted.The exosomes were used to intervene the endothelial cell proliferation inhibition model induced by rapamycin.The cell proliferation ability of each group was measured by MTT method.The expression of PCNA and Ki67 in cells was measured by Western blot.The gene chip was used to detect the difference in non-coding RNA of the two groups of exosomes,and qRT-PCR was used to detect the expression of lnc HOXA11-AS.Results:Compared with the control group,the cell proliferation ability of the rapamycin group was weakened,and the expression of PCNA and Ki67 decreased(P<0.05);compared with the rapamycin group,the endothelial cell proliferation ability of the control group increased after exosomal intervention,and the expression of PCNA and Ki67 increased(P<0.05).The microarray results showed that the expression of lnc HOXA11-AS was reduced in ISR exosomes.pHOXA11-AS increased the proliferation of endothelial cells as well as the expression of PCNA,Ki67 and SOX4.Conclusion:Lnc HOXA11-AS in plasma exosomes may increase the proliferation ability of endothelial cells by up-regulating the expression of SOX4.

关 键 词：支架内再狭窄 外泌体 HOXA11-AS 

分 类 号：R914[医药卫生—药物化学]