靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究  

Application of Targeted Proteomics in Species Identification of Milk Proteins in Multi-species Milk Products

作　　者：陈雨湉 任一平 王丽丽[1] 黄忠平[1] CHEN Yu-Tian;REN Yi-Ping;WANG Li-Li;HUANG Zhong-Ping(College of Chemical Engineering,Zhejiang University of Technology,Hangzhou 310014,China;Yangtze Delta Region Institute of Tsinghua University,Zhejiang Analysis and Test Center,Jiaxing 314006,China)

机构地区：[1]浙江工业大学化学工程学院,杭州310014 [2]清华长三角研究院分析测试中心,嘉兴314006

出　　处：《分析化学》2021年第9期1523-1530,I0003-I0013,共19页Chinese Journal of Analytical Chemistry

摘　　要：基于靶向蛋白质组学技术,采用超高效液相色谱-串联高分辨质谱/串联三重四极杆质谱,建立了一种鉴别奶牛、水牛、牦牛、山羊、绵羊、马、驴和骆驼共8个物种奶的分析方法。针对8个物种奶中6种乳蛋白酶解产生的特征多肽进行分析,利用同位素内标法实现多条多肽的同时检测及比较,通过理论多肽分析、实际多肽检测、干扰考察、线性与最大酶解时间比较,最终筛选出13条特征多肽。依据13条特征多肽对模拟混合奶样品中的不同物种奶进行分析,对市售16个奶制品中的奶源物种进行鉴别。结果表明,特征多肽在奶源物种鉴别中具有良好的稳定性与特异性,本方法对多物种奶制品行业的质量控制与监管具有参考价值。A method based on target proteomics and ultrahigh performance liquid chromatography-high resolution mass spectrometry/tandem mass spectrometry was established for species identification of milks and dairies from cow,buffalo,yak,goat,sheep,horse,donkey and camel.Tryptic hydrolyzed peptides from 6 kinds of milk proteins in 8 species were analyzed and screened through theoretical analysis,instrument examination,investigation of interferences,linearity and digestion speed.A total of 13 specific peptides were finally chosen and applied to identification and detection of multi-species for milk mixture samples and commercial dairies.The results showed that specific peptides maintained good stability and specificity during species analysis.This method provided valuable suggestion in quality control and supervision of multi-species diary industry.

关 键 词：靶向蛋白质组学 液相色谱-质谱联用技术 物种鉴别 乳蛋白 

分 类 号：O657.63[理学—分析化学] TS252.7[理学—化学]