肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析  

Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma

作　　者：郭佳[1] 魏丽萍 陈志营[1] 何成彦[2] GUO Jia;WEI Li-Ping;CHEN Zhi-Ying;HE Cheng-Yan(Department of Respiratory,China-Japan Union Hospital of Jilin University,Changchun 130033,China;Department of Clinical Laboratory,China-Japan Union Hospital of Jilin University,Changchun 130033,China)

机构地区：[1]吉林大学中日联谊医院呼吸科,长春130033 [2]吉林大学中日联谊医院检验科,长春130033

出　　处：《分析化学》2021年第9期1563-1571,共9页Chinese Journal of Analytical Chemistry

基　　金：吉林省科技厅项目(Nos.20190201215JC,20200404193YY)资助。

摘　　要：肾透明细胞癌中介导蛋白质棕榈酰化修饰的蛋白酰基转移酶表达异常,提示肾透明细胞癌可能存在蛋白质棕榈酰化修饰异常改变,并与肿瘤代谢紊乱或致癌信号通路的激活密切相关。本研究采用优化的酰基-生物素置换法(Acyl-biotinyl exchange,ABE),结合蛋白质组学技术,对肾透明细胞癌组织中S-棕榈酰化修饰蛋白进行全面分析,共鉴定出92种高可信度的棕榈酰化修饰蛋白,包括细胞骨架相关蛋白4、磷酸丙糖异构酶、脂肪分化相关蛋白、膜联蛋白A4、微管相关蛋白tau蛋白和钙联蛋白等。通过蛋白质印迹分析技术对其中的脂肪分化相关蛋白(Adipose differentiation-related protein,ADRP)棕榈酰化修饰进行了验证。本研究提供了一种改进的适合分析组织样本的ABE实验方法,所得肾透明细胞癌组织中的S-棕榈酰化修饰蛋白的分析结果可为肾透明细胞癌早期诊断、治疗和预后的分子靶点等方面的研究提供基础数据,对进一步深入研究肾透明细胞癌发病机制具有重要的参考价值。The abnormal expression of protein acyltransferase that is responsible for protein palmitoylation in renal clear cell carcinoma suggests that there may be abnormal changes of S-palmitoylation in renal clear cell carcinoma,which is closely related to metabolic disorder or activation of carcinogenic signaling pathway.In the present study,the optimized acyl-biotinyl exchange(ABE)method combined with proteomic techniques was used to analyze S-palmitoylated proteins in clear cell renal cell carcinoma.A total of 92 palmitoylated proteins were identified with high reliability,and the S-palmitoylation of adipose differentiation related protein(ADRP)was verified by Western blotting.This study provides an improved ABE method suitable for analyzing tissue samples.The identification of palmitoylated proteins in clear cell renal cell carcinoma can provide primary data for the molecular targets of early diagnosis,treatment and prognosis of clear cell renal carcinoma,which are of great significance for further study of the pathogenesis of clear cell renal carcinoma.

关 键 词：S-棕榈酰化修饰 肾透明细胞癌 蛋白质组 酰基-生物素置换法 脂肪分化相关蛋白 

分 类 号：R737.11[医药卫生—肿瘤]