高效液相色谱-串联质谱法测定蛋和奶中氨基糖苷类药物残留  

Determination of aminoglycoside residues in eggs and milk by high-performance liquid chromatography-tandem mass spectrometry

作　　者：栾枫婷 龚兰 朱磊[1,2] 陈明 唐敏敏[1,2] 邵雪梅 魏瑞成[1,2] 王冉 LUAN Feng-ting;GONG Lan;ZHU Lei;CHEN Ming;TANG Min-min;SHAO Xue-mei;WEI Rui-cheng;WANG Ran(Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base,Nanjing 210014,China;Institute of Food Safety and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地,江苏南京210014 [2]江苏省农业科学院农产品质量安全与营养研究所,江苏南京210014

出　　处：《江苏农业学报》2021年第4期1033-1042,共10页Jiangsu Journal of Agricultural Sciences

摘　　要：建立了高效液相色谱-串联质谱法测定蛋和奶中氨基糖苷类药物(双氢链霉素、卡那霉素A、庆大霉素、链霉素、妥布霉素、潮霉素B、大观霉素、阿米卡星、新霉素B和安普霉素)残留。用5%三氯乙酸水溶液提取目标物,经HLB固相萃取柱净化,高效液相色谱柱分离,梯度洗脱,外标法定量。结果表明,10种药物在各自范围内线性关系良好,R2>0.99,方法的检出限为10.0~50.0μg/kg,定量限为30.0~100.0μg/kg,平均回收率为62.3%~110.4%,相对标准偏差为1.0%~13.6%。该方法简便、准确、灵敏,且扩大了检测范围,适用于蛋和奶中氨基糖苷类药物的定量测定。The high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)was developed for the simultaneous determination of ten aminoglycosides(dihydrostreptomycin,kanamycin A,gentamycin,streptomycin,spectinomycin,tobramycin,hygromycin B,amikacin,neomycin B and apramycin)in milk and eggs.The samples were extracted with 5%trichloroacetic acid solution,followed by Oasis HLB solid phase extraction for further purification.The analytes were separated on a high performance liquid chromatography column,then analyzed by MS/MS with electrospray ionization in positive mode,and quantified by external standard method.Results showed that there were good linear relationships for 10 target analytes,and the linear correlation coefficients were greater than 0.99.The limits of detection and the limits of quantitation were in the ranges of 10.0-50.0μg/kg and 30.0-100.0μg/kg,respectively.The average recoveries for ten aminoglycosides ranged from 62.3%to 110.4%with relative standard deviations of 1.0%-13.6%.The developed method is simple,rapid,accurate,sensitive and has wide detection range,so it is suitable for the determination of ten aminoglycosides in eggs and milk.

关 键 词：高效液相色谱-串联质谱 固相萃取 氨基糖苷类药物 残留 禽蛋 奶 

分 类 号：S859.84[农业科学—临床兽医学]