莞香茶黄酮类化合物体外抗氧化活性的探究  

Antioxidant Activity of Flavonoids from Guanxiang Tea in Vitro

作　　者：刘红文 戴昕彤 何权 黄娟 刘雨果 李林秋 Liu Hongwen;Dai Xintong;He Quan;Huang Juan;Liu Yuguo;Li Linqiu(Marine Biomedical Research Institute of Guangdong Medical University,Zhanjiang 524023,China;School of Public Health,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学海洋医药研究院南海海洋生物医药资源研发公共服务平台,广东湛江524023 [2]广东医科大学公共卫生学院,广东东莞523808

出　　处：《山东化工》2021年第15期52-54,57,共4页Shandong Chemical Industry

基　　金：广东医科大学博士启动项目(B2017022);广东医科大学科研基金(2XK19023);广东医科大学公共卫生与预防医学学科发展基金(4SG20004G);广东医科大学大学生创新实验项目(ZYDG004)。

摘　　要：以抗坏血酸为阳性对照,建立ABTS^(+)自由基、DPPH自由基、Cu^(2+)还原力和总体外抗氧化模型考察两种品牌莞香茶黄酮类化合物的体外抗氧化活性。结果显示,莞香茶1黄酮类化合物清除ABTS^(+)·、DPPH·和还原Cu^(2+)的IC50分别为(5.651±0.031),(8.633±0.064),(4.450±0.096)μg/mL,莞香茶2的IC50则分别为(4.752±0.216),(5.465±0.047),(4.639±0.066)μg/mL;莞香茶1、莞香茶2与维生素C的FRAP值分别为0.218,0.279与0.180 mmol/L。莞香茶黄酮类化合物具有与维生素C相当的强体外抗氧化活性,与其质量浓度存在一定的量效关系,且莞香茶2强于莞香茶1。The in vitro antioxidant activity of flavonoids from Guanxiang Tea was studied by determining the scavenging capacity of ABTS^(+)·,DPPH·,reducing capcity of Cu^(2+)and the ferric reducing ability of plasma with vitamin C as positive control.The results showed the half inhibitory mass concentration(IC50)of flavonoids from Xianglaoli Tea on ABTS^(+)·,DPPH·and Cu^(2+)were(5.651±0.031),(8.633±0.064),(4.450±0.096)μg/mL,respectively.The IC50 of flavonoids from Hejiaren Tea on ABTS^(+)·,DPPH·and Cu^(2+)were(4.752±0.216),(5.465±0.047),(4.639±0.066)μg/mL,respectively.The FRAP of Xianglaoli Tea,Jiarene Tea and vitamin C were 0.218,0.279,0.180 mmol/L,respectively.The flavonoids of Guanxiang tea have strong antioxidant activity comparable to vitamin C in vitro.There is a dose-effect relationship between the antioxidant activity and the mass concentration of flavonoids,and the antioxidant activity of Jiarene Tea is stronger than Xianglaoli Tea.

关 键 词：莞香茶 黄酮类化合物 抗氧化活性 

分 类 号：TQ461[化学工程—制药化工]