Enhancing the treatment effects of tumor cell purified autogenous heat shock protein 70-peptide complexes on HER-3-overexpressing breast cancer  

作　　者：Xia Chen Xiaoming Zhang Xiangji Lu Meng Ren Rina Su Weishi Gao Yanwei Gao 

机构地区：[1]Department of Apheresis,Inner Mongolia Red Cross Blood Center,Hohhot,Inner Mongolia 010010,China [2]Department of Ultrasound medicine,Inner Mongolia People’s Hospital,Hohhot,Inner Mongolia 010017,China [3]Department of General Surgery,Inner Mongolia Armed Police Hospital,Hohhot,Inner Mongolia 010010,China [4]Department of Surgical Oncology,Inner Mongolia People’s Hospital,Hohhot,Inner Mongolia 010017,China

出　　处：《Oncology and Translational Medicine》2021年第4期165-171,共7页肿瘤学与转化医学（英文版）

基　　金：Supported by a grant from the National Natural Science Foundation of China(No.81260392).

摘　　要：Objective The aim of this study was to enhance the treatment effect of tumor purified autogenous heat shock protein 70-peptide complexes(HSP70-PCs)on HER-3-overexpressing breast cancer.Methods In this study,we first studied the expression of HER-3 in breast cancer tissues and its relationship with patient characteristics.We then purified HSP70-PCs from primary breast cancer cells with different HER-2 and HER-3 expression profiles and determined the cytotoxicity of autogenous dendritic cells(DCs)and CD8+T cells induced by these complexes.Third,recombinant human HSP70-HER-3 protein complexes were used to inhibit the autogenous HSP70-PCs purified from HER-3-overexpressing breast cancer cells,and the resulting immunological response was examined.Results The results show that HSP70-PCs can be combined with recombinant HSP70-HER-3 protein complexes to induce stronger immunological responses than autogenous HSP70-PCs alone and that these treatments induce autogenous CD8+T cell killing of HER-3-positive breast cancer cells.Conclusion These findings provide a new direction for HSP70-DC-based immunotherapy for patients with HER-3-overexpressing breast cancer.

关 键 词：heat shock protein 70 peptide complexes(HSP70-PCs) HER-3 protein recombinant protein dendritic cells(DCs) cellular immunotherapy 

分 类 号：R737.9[医药卫生—肿瘤]