汞离子比色型荧光探针的合成与性质  

Synthesis and properties of colorimetric fluorescent probe for mercury ions

作　　者：管怡晗 黎广进 刘盛华[1] 尹军[1] GUAN Yihan;LI Guangjin;LIU Shenghua;YIN Jun(Central China Normal University,College of Chemistry,Key Laboratory of Pesticide&Chemical Biology of Ministry of Education,Wuhan,430000,China)

机构地区：[1]华中师范大学化学学院,农药与化学生物学教育部重点实验室,武汉430000

出　　处：《环境化学》2021年第8期2544-2550,共7页Environmental Chemistry

基　　金：国家自然科学基金(21676113,21772054);湖北省杰出青年基金(18CFA079);华中师范大学学者名师支持项目(0900-31101090002);华中师范大学高等院校基础研究与运营优秀博士学位论文培养经费(2019YBZZ029)资助.

摘　　要：汞化合物在促进工业和制造业快速发展的同时,也带来了严重的环境污染。由于其毒性极强,易于渗透,难以代谢,在生物体内富集后容易造成长期伤害。因此,开发一种能够快速、简洁、准确检测汞离子的方法具有十分重要的意义。为此,本文发展了一个基于氧硫杂冠醚配体的汞离子特异性的比色型荧光探针。该探针能够通过比色及荧光高选择性检测汞离子,且干扰性小、灵敏快捷。探针可以与汞离子形成1:1络合物,该络合物可以在碘离子作用下将汞离子从配合物中解离,利用该平衡可以对汞离子进行定量检测。特别值得提及的是,该探针能够用于细胞及活体中汞离子的可视化检测。While mercury compounds promote the rapid development of industry and manufacturing industry,they also bring serious environmental pollution.Due to its high toxicity,easy penetrability and difficult metabolism,it is easy to cause long-term damage after aggregation in organisms.Therefore,it is of great significance to develop a rapid,simple and accurate method to detect mercury ions.Accordingly,a mercury-ion specific colorimetric fluorescent probe based on oxy-thio-crown ether ligand was developed.The probe could detect mercury ions through colorimetry and fluorescence with high selectivity,low interference,high sensitivity and quick response time.Moreover,the formed 1:1 complex between probe and mercury ions could be dissociated in the presence of iodide ions,and the equilibrium could be applied in quantitative detection of mercury ions.In particular,the probe could be used to track mercury ions in living cells and in vivo.

关 键 词：荧光探针 汞离子 比色传感 生物成像 

分 类 号：O657.3[理学—分析化学]