1株栓孔菌的分离鉴定、生物学特性及胞内多糖抗氧化性研究  

Isolation and identification,biological characteristics and intracellular polysaccharides antioxidant activity of a Trametes strain

作　　者：张鹏 骆琴[1] 唐森 覃逸明 蒋才云[1,2] ZHANG Peng;LUO Qin;TANG Sen;QIN Yi-ming;JIANG Cai-yun

机构地区：[1]广西科技师范学院食品与生化工程学院,广西来宾546199 [2]广西科技师范学院特色瑶药资源研究与开发重点实验室,广西来宾546199 [3]广西科技师范学院科研管理处,广西来宾546199

出　　处：《饲料研究》2021年第14期93-98,共6页Feed Research

基　　金：2018年度广西高校中青年教师基础能力提升项目(项目编号:2018KY0702);广西科技师范学院重点科研项目资助(项目编号:GXKS2020ZD002);2018-2020年广西本科高校食品科学与工程特色专业及实验实训教学基地(中心)建设项目(项目编号:桂教高教[2018]52号)。

摘　　要：试验对分离自栓孔菌子实体的1株大型真菌进行鉴定,分析其生物学特性,并测定菌丝体胞内多糖的抗氧化性。结果显示,菌株TL2019为奶油栓孔菌(Trametes lactinea)。菌丝生长的最适条件为碳源为葡萄糖,氮源为牛肉膏,生长因子为维生素C,pH值7.0,生长温度28℃。经测定胞内多糖提取量为13.32 mg/g,胞内多糖对羟基自由基(IC50=0.64 g/L)和超氧阴离子自由基(IC50=0.41 g/L)具有一定的清除活性。A large fungus strain isolated from the fruiting body of Trametes sp.was identified,the biological characteristics were analyzed,and the antioxidant activity of Intracellular polysaccharides was determined.The results showed that strain TL2019 was identified as Trametes lactinea.The optimum conditions for mycelium growth were glucose as carbon source,beef paste as nitrogen source,vitamin C as growth factor,pH value 7.0 and 28℃ as growth temperature.The extraction amount of intracellular polysaccharide was 13.32 mg/g,and it had certain scavenging activity against hydroxyl radical(IC_(50)=0.64 g/L)and superoxide anion radical(IC_(50)=0.41 g/L).

关 键 词：奶油栓孔菌 液体发酵 胞内多糖 分离鉴定 抗氧化性 

分 类 号：S816.3[农业科学—饲料科学]