还原型谷胱甘肽修饰的金纳米团簇荧光传感器对铜离子的高灵敏检测  

Highly Sensitive Detection of Copper Ions by Reduced Glutathione Modified Gold Nanocluster Fluorescence Sensor

作　　者：刘振平[1] 姜容[1] 庞钶靖 Liu Zhenping;Jiang Rong;Pang Kejing(Chongqing Vocational Institute of Safety&Technology,Chongqing 404020,China)

机构地区：[1]重庆安全技术职业学院,重庆404020

出　　处：《激光与光电子学进展》2021年第14期104-112,共9页Laser & Optoelectronics Progress

基　　金：重庆市教育委员会科学技术研究计划青年项目(KJQN201804703)。

摘　　要：以还原型谷胱甘肽(GSH)为稳定剂合成具有较好的稳定性和水溶性的金纳米团簇(GSH-AuNCs),在水溶液中利用Cu^(2+)对GSH-AuNCs荧光的特异性静态猝灭效应建立Cu^(2+)快速且高灵敏的检测方法。GSH-AuNCs在396 nm波长的激发光照射下,在602 nm处发射出较强的荧光,当GSH-AuNCs体系中存在Cu^(2+)时,荧光信号会在一定程度上猝灭,且Cu^(2+)浓度的越大猝灭效应越显著,据此建立GSH-AuNCs荧光发射强度与Cu^(2+)浓度的线性关系,实现对Cu^(2+)的定量检测。在优化的条件下,GSH-AuNCs荧光发射信号变化与Cu^(2+)浓度在16.7~5000 nmol·L^(-1)之间呈良好的线性关系,检出限为7.4 nmol·L^(-1)。同时,对实际水样品进行加标回收率实验,回收率在92.9%~107.9%之间。Reducing glutathione(GSH)is used as a stabilizer to synthesize gold nanoclusters(GSH-AuNCs)with good stability and water solubility.The specific static quenching effect of Cu^(2+)on GSH-AuNCs fluorescence in aqueous solution is used to establish a rapid and highly sensitive detection method for Cu^(2+).GSH-AuNCs emits strong fluorescence at 602nm under the excitation light of 396nm wavelength.When Cu^(2+)exists in the GSH-AuNCs system,the fluorescence signal is quenched to a certain extent,and the greater the concentration of Cu^(2+),the more significant the effect,the linear relationship between the fluorescence emission intensity of GSH-AuNCs and the concentration of Cu^(2+)is established based on this,and the quantitative detection of Cu^(2+)is realized.Under optimized conditions,the fluorescence emission signal of GSH-AuNCs showes a good linear relationship with Cu^(2+)concentration between 16.7and 5000nmol·L^(-1),and the detection limit is 7.4nmol·L^(-1).At the same time,the recovery rate of the actual water sample is detected,the recovery rate is between 92.9%and 107.9%.

关 键 词：探测器 还原型谷胱甘肽 金纳米团簇 荧光传感器 铜离子 水 

分 类 号：O433.4[机械工程—光学工程]