Erastin诱导人非小细胞肺癌细胞系A549凋亡  

Erastin induces cell apoptosis in human non-small cell lung cancer cell line A549

作　　者：姜学东 李文雅[2] JIANG Xue-dong;LI Wen-ya(Department of Thoracic Surgery,Liaoning Provincial Corps Hospital,Chinese People s Armed Police Forces,Shenyang 110034;Department of Thoracic Surgery,the First Affiliated Hospital,China Medical University,Shenyang 110001,China)

机构地区：[1]中国人民武装警察部队辽宁省总队医院胸外科,辽宁沈阳110034 [2]中国医科大学附属第一医院胸外科,辽宁沈阳110001

出　　处：《基础医学与临床》2021年第9期1313-1317,共5页Basic and Clinical Medicine

摘　　要：目的探讨爱拉斯汀(erastin)对非小细胞肺癌细胞系A549凋亡的诱导作用及机制。方法不同浓度的erastin处理A549细胞,加入/不加入活性氧集团(ROS)清除剂N-乙酰基-L-半胱氨酸(NAC),加入/不加入凋亡抑制剂(Z-VAD-FMK);试剂盒检测细胞铁含量;CCK-8法和流式细胞测量术检测细胞损伤和凋亡等。结果Erastin引起A549细胞铁离子含量升高和细胞损伤,诱导细胞凋亡(P<0.05),该作用可被凋亡caspase抑制剂Z-VAD-FMK抑制;Erastin引起过氧化物(ROS)增加从而诱导细胞凋亡(P<0.05),该作用可被ROS清除剂NAC减弱(P<0.05)。结论Erastin通过诱导非小细胞肺癌细胞系A549内ROS产生,引起细胞凋亡,该发现为非小细胞肺癌的诊疗策略提供了参考。Objective To explore whether erastin induces apoptosis and to investigate the molecular mechanism in A549 cells.Methods A549 cells were treated with erastin at different concentrations,with or without ROS scavenging agent N-acetyl-L-cysteine(NAC)and with or without apoptosis inhibitor(Z-VAD-FMK).Then iron content was measured with Iron Assay Kit,cell injure and apoptosis with CCK-8 and flow cytometry in A549 cells.Results Erastin induced the increase of iron content and followed by the injury of A549 cells.Erastin further induced apoptosis of A549 cells(P<0.05),which was inhibited by apoptosis inhibitor Z-VAD-FMK.Erastin induced an increase in peroxides(ROS)then followed by apoptosis(P<0.05),which was inhibited by the ROS scavenging agent NAC(P<0.05).Conclusions Erastin has an inhibitory effect on NSCLC A549 by inducing intracellular ROS production,which provides evidences for the pathogenesis,diagnosis and treatment of NSCLC.

关 键 词：爱拉斯汀 非小细胞肺癌 凋亡 

分 类 号：R394-33[医药卫生—医学遗传学]