高脂饲养C57BL/6J小鼠肾周脂肪组织炎性反应的评估  

Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet

作　　者：杨永 李梓玉 杨伟 刘勇 YANG Yong;LI Zi-yu;YANG Wei;LIU Yong(Department of Pathology,the Affiliated Hospital of Southwest Medical University,Luzhou 646000;Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province,Luzhou 646000,China)

机构地区：[1]西南医科大学附属医院病理科,四川泸州646000 [2]核医学与分子影像四川省重点实验室,四川泸州646000

出　　处：《基础医学与临床》2021年第9期1318-1322,共5页Basic and Clinical Medicine

基　　金：西南医科大学附属医院博士科研启动基金(20012);西南医科大学附属医院科研项目(17128);核医学与分子影像四川省重点实验室2020年开放课题(2020-206)。

摘　　要：目的评估高脂饲养C57BL/6J小鼠肾周脂肪组织炎性反应。方法将小鼠随机分为对照组(control组,n=6)和高脂饲养组(HFD组,n=6)。用RT-qPCR检测肾周脂肪组织中TNF-α、CD11c、IL-1β、IL-10、TGF-β1、CD206等mRNA的表达;免疫组织化学法检测肾周脂肪组织及肾实质F4/80、CD68、LCA的水平。结果与对照组比较,HFD组小鼠肾周脂肪组织TNF-α、IL-1β等mRNA相对含量升高(P<0.05);HFD组的肾周脂肪巨噬细胞标志物F4/80,CD68及炎细胞广谱标志物LCA的表达均呈高表达(P<0.05)。结论高脂饲养的C57BL/6J小鼠肾周脂肪组织确实存在明显的炎性反应。Objective To evaluate the inflammation of perirenal adipose tissue induced by high-fat diet in C57BL/6J mice.Methods Mice were randomly divided into normal diet group(control group,n=6)and high-fat diet group(HFD group,n=6).The mRNA expression of TNF-α,CD11c,IL-1β,IL-10,TGF-β1,CD206 in perirenal adipocytes were detected by RT-qPCR.The expressions of F4/80,CD68 and LCA in perirenal adipose tissue and renal parenchyma were detected by immunohistochemistry method.Results Compared with the control group,the mRNA expression by TNF-αand IL-1βin perirenal adipose tissue of mice in HFD group was increased(P<0.05);The expression of F4/80,CD68 and LCA in perirenal fat increased in HFD group(P<0.05).Conclusions High-fat diet induces inflammation in perirenal adipose tissue of C57BL/6J mice.

关 键 词：脂肪组织 高脂饲养 炎性反应 肾 巨噬细胞 

分 类 号：R364.5[医药卫生—病理学]