油茶叶多糖的闪式提取工艺优化及其抗氧化活性  

Optimization of Flash Extraction Technology of Polysaccharide from Leaves of Camellia oleifera Able.and Its Antioxidant Activity

作　　者：叶润 蔡静 李尽哲 孙伟 申家朋 YE Run;CAI Jing;LI Jinzhe;SUN Wei;SHEN Jiapeng(School of Biology and Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China;Forestry Institute,Xinyang Agriculture and Forestry University,Xinyang 464000,China)

机构地区：[1]信阳农林学院生物与制药工程学院,河南信阳464000 [2]信阳农林学院林学院,河南信阳464000

出　　处：《生物质化学工程》2021年第5期42-46,共5页Biomass Chemical Engineering

基　　金：河南省科技攻关项目(172102310425);河南省教育厅项目(20B180012);校青年基金项目(2019LG001)。

摘　　要：在单因素试验基础上用响应面法对油茶叶多糖的闪式提取工艺进行优化,并对较优条件下提取的多糖的抗氧化活性进行测试。研究结果表明:油茶叶多糖闪式提取的较佳工艺条件为料液比1∶30(g∶mL),提取温度81℃,提取时间75 s,此条件下,油茶叶多糖得率为8.43%。油茶叶多糖对DPPH·、·ABTS+、OH·都有很强的清除能力,其半数抑制浓度(IC50)值分别为1.706、0.826和4.811 g/L,表现出很好的抗氧化活性。On the basis of single factor experiment,the flash extraction process of polysaccharide from the leaves of Camellia oleifera Able.was optimized by response surface methodology,and the antioxidant activity of polysaccharide was investigated.The optimum conditions of flash extraction were as follows:material-liquid ratio 1∶30(g∶mL),temperature 81℃,extraction time 75 s.Under these conditions,the extraction rate of polysaccharide was 8.43%.The polysaccharide from the leaves of Camellia oleifera Able.had strong scavenging ability to DPPH·,·ABTS+and hydroxyl radicals,with the inhibitory concentration 50%(IC50)values of 1.706,0.826 and 4.811 g/L,respectively.Therefore,it had good antioxidant activity.

关 键 词：油茶叶 多糖 响应面法 抗氧化活性 

分 类 号：TQ35[化学工程] O629.3[理学—有机化学]