橄榄苦苷对人前列腺癌PC-3细胞增殖、迁移侵袭及凋亡的影响  

Effect of Oleuropein on the Proliferation,Migration and Apoptosis in Human Prostatic Cancer PC-3 Cells

作　　者：田凤 龙梦晖 宁志丰 佘同辉 TIAN Feng;LONG Meng-hui;SHE Tong-hui(School of Pharmacy,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院药学院,湖北咸宁437100 [2]湖北科技学院基础医学院

出　　处：《湖北科技学院学报（医学版）》2021年第4期289-291,F0002,共4页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院五官医学院专项科研基金(2020WG09)。

摘　　要：目的探讨橄榄苦苷对人前列腺癌PC-3细胞增殖、迁移侵袭和凋亡的影响及可能机制。方法以不同浓度橄榄苦苷(0、20、40、80、160、320μmol/L)处理PC-3细胞,采用MTT实验检测PC-3细胞的增殖活性、划痕实验检测细胞迁移、Transwell侵袭实验检测细胞侵袭能力,以及Western blot检测PC-3细胞Bcl-2、Bad蛋白的表达。结果橄榄苦苷可剂量依赖性地抑制PC-3细胞的增殖、迁移与侵袭;PC-3细胞中抗凋亡蛋白Bcl-2的表达有下调趋势,而促凋亡蛋白Bad的表达有上调趋势,且均呈浓度依赖性。结论橄榄苦苷剂量依赖性抑制PC-3细胞的增殖与迁移侵袭,并通过调节Bcl-2、Bad表达促进PC-3细胞凋亡。Objective To investigate the effects of oleuropein on the proliferation,migration and apoptosis of human prostatic cancer PC-3 cells and its possible mechanism.Methods PC-3 cells were treated with different concentrations of oleuropein(0、20、40、80、160、320μmol/L).The proliferation activity of PC-3 cells was detected by MTT assay,cell migration was detected by scratch assay,and cell invasion ability was detected by Transwell invasion assay.Western blot was used to detect the expression of Bcl-2 and Bad proteins in PC-3 cells.Results Oleuropein can inhibit the proliferation,migration and invasion of PC-3 cells in a dose-dependent manner.Meanwhile,Western blot analysis showed that the expression of anti-apoptotic protein Bcl-2 decreased in PC-3 cells,while the expression of pro-apoptotic protein Bad increased in a concentration-dependent manner.Conclusion Oleuropein dose-dependently inhibited the proliferation,migration and invasion of PC-3 cells,and promoted the apoptosis of PC-3 cells by regulating the expression of Bcl-2 and Bad.

关 键 词：橄榄苦苷 前列腺癌 增殖 迁移 凋亡 

分 类 号：R73[医药卫生—肿瘤]