Experimental nerve transfer model in the neonatal rat  

作　　者：Matthias E.Sporer Martin Aman Konstantin D.Bergmeister Dieter Depisch Katharina M.Scheuba Ewald Unger Bruno K.Podesser Oskar C.Aszmann 

机构地区：[1]Christian Doppler Laboratory for the Restoration of Extremity Function,Department of Surgery,Medical University of Vienna,Vienna,Austria [2]Clinical Laboratory for Bionic Extremity Reconstruction,Department of Surgery,Medical University of Vienna,Vienna,Austria [3]Division of Biomedical Research,Medical University of Vienna,Vienna,Austria [4]Department of Plastic,Aesthetic and Reconstructive Surgery,University Hospital of St.Poelten,Karl Landsteiner University of Health Sciences,St.Poelten,Austria [5]Center for Medical Physics and Biomedical Engineering,Medical University of Vienna,Vienna,Austria [6]Division of Plastic and Reconstructive Surgery,Medical University of Vienna,Vienna,Austria

出　　处：《Neural Regeneration Research》2022年第5期1088-1095,共8页中国神经再生研究（英文版）

基　　金：supported by the Christian Doppler Research Association and the European Research Council under the European Union’s Horizon 2020 research and innovation program(both to OCA)。

摘　　要：Clinically,peripheral nerve reconstructions in neonates are most frequently applied in brachial plexus birth injuries.Most surgical concepts,however,have investigated nerve reconstructions in adult animal models.The immature neuromuscular system reacts differently to the effects of nerve lesion and surgery and is poorly investigated due to the lack of reliable experimental models.Here,we describe an experimental forelimb model in the neonatal rat,to study these effects on both the peripheral and central nervous systems.Within 24 hours after birth,three groups were prepared:In the nerve transfer group,a lesion of the musculocutaneous nerve was reconstructed by selectively transferring the ulnar nerve.In the negative control group,the musculocutaneous nerve was divided and not reconstructed and in the positive control group,a sham surgery was performed.The animal's ability to adapt to nerve lesions and progressive improvement over time were depict by the Bertelli test,which observes the development of grooming.Twelve weeks postoperatively,animals were fully matured and the nerve transfer successfully reinnervated their target muscles,which was indicated by muscle force,muscle weight,and cross sectional area evaluation.On the contrary,no spontaneous regeneration was found in the negative control group.In the positive control group,reference values were established.Retrograde labeling indicated that the motoneuron pool of the ulnar nerve was reduced following nerve transfer.Due to this post-axotomy motoneuron death,a diminished amount of motoneurons reinnervated the biceps muscle in the nerve transfer group,when compared to the native motoneuron pool of the musculocutaneous nerve.These findings indicate that the immature neuromuscular system behaves profoundly different than similar lesions in adult rats and explains reduced muscle force.Ultimately,pathophysiologic adaptations are inevitable.The maturing neuromuscular system,however,utilizes neonatal capacity of regeneration and seizes a variety of compensation mecha

关 键 词：brachial plexus birth injury experimental rat model extremity reconstruction methodological paper neonatal rat nerve reconstruction nerve regeneration nerve transfer neural plasticity peripheral nerve surgery 

分 类 号：R-332[医药卫生] R722.1