细胞因子Irisin缓解高糖诱导骨髓间充质干细胞凋亡的机制研究  

Mechanisms of irisin attenuates cell apoptosis of bone-marrow mesenchymal stem cells in high glucose condition

作　　者：郭树琴 焦婷[3] 赵立升 杨烁[2] 党瑞杰[2] 温宁[2] 朱彪 GUO Shuqin;JIAO Ting;ZHAO Lisheng;YANG Shuo;DANG Ruijie;WEN Ning;ZHU Biao(Department of Stomatology,Beijing Railway Construction Hospital,Beijing 100855,China;Department of Stomatology,the First Medical Center,Chinese PLA General Hospital,Beijing 100853,China;Oncology Division,Affiliated Hospital of Hebei University,Baoding 071000,Hebei Province,China;Department of Stomatology,Fuxing Hospital,Capital Medical University,Beijing 100038,China)

机构地区：[1]北京铁建医院口腔科,北京100855 [2]解放军总医院第一医学中心口腔科,北京100853 [3]河北大学附属医院肿瘤科,河北保定071000 [4]首都医科大学附属复兴医院口腔科,北京100038

出　　处：《解放军医学院学报》2021年第6期629-633,共5页Academic Journal of Chinese PLA Medical School

基　　金：中国博士后科学基金资助项目(2019T120977)。

摘　　要：背景高糖可诱导骨髓间充质干细胞(bone-marrow mesenchymal stem cells,BMSCs)凋亡,而细胞因子Irisin对糖毒性所致的细胞损伤具有保护作用。目的探讨细胞因子Irisin对高糖环境中BMSCs凋亡的影响及其作用机制。方法复苏BMSCs并将其分为对照组(Vehicle)、高糖诱导组(high glucose,HG)、Irisin干预组(HG+irisin;Irisin)和抑制剂组(HG+irisin+compound C,CC)。各组细胞培养3 d后,流式细胞术检测细胞凋亡率。Western blotting检测凋亡相关蛋白Bcl-2/Bax比值和磷酸化AMP依赖的蛋白激酶(phosphorylated AMP-activated protein kinase,p-AMPK)、AMPK的表达水平。结果与Vehicle组相比,HG组BMSCs细胞凋亡率显著升高(P<0.05);Irisin可降低高糖诱导的BMSCs细胞凋亡(P<0.05),提高Bcl-2/Bax的蛋白表达比(P<0.05),同时激活AMPK。当特异性地抑制AMPK后,Irisin缓解高糖诱导BMSCs凋亡的作用明显减弱。结论Irisin可通过激活AMPK缓解高糖诱导的BMSCs凋亡。Background Previous studies revealed that high glucose could induce bone-marrow mesenchymal stem cells apoptosis,and irisin attenuated cell injury resulted from glucotoxicity.Objective To investigate the effects of irisin on cell apoptosis of bone-marrow mesenchymal stem cells(BMSCs)in high glucose condition.Methods BMSCs were divided into Vehicle group,HG(high glucose)group,Irisin(HG plus irisin intervention)group,and CC[(HG+irisin+Compound C,CC)]group.After cultured for 3 days,flow cytometry was used to evaluate apoptotic rate,and Western blotting was used to investigate protein level of Bcl-2/Bax and phosphorylated AMP-activated protein kinase(p-AMPK).Results Compared with the Vehicle group,apoptotic rate of HG group increased obviously(P<0.05);while Irisin intervention could reduce apoptotic rate(P<0.05),increase Bcl-2/Bax expression(P<0.05),and activate AMPK.However,when inhibiting AMPK,the effects of Irisin on inhibiting cell apoptosis were blunted(P<0.05).Conclusion Irisin can inhibit cell apoptosis of BMSCs in high glucose condition via activating AMPK.

关 键 词：Irisin 细胞因子 糖尿病 骨髓间充质干细胞 细胞凋亡 

分 类 号：R780.2[医药卫生—口腔医学]