蜂王幼虫冻干粉对PCPA失眠小鼠的改善作用  

Effect of Lyophilized Powder of Queen Bee Larva on PCPA Insomnia Mice

作　　者：唐启河 廖世群 王佳炫 萧杨 陈奕霏 曹喆 田文礼[2] 郭军[1] TANG Qi-he;LIAO Shi-qun;WANG Jia-xuan;XIAO Yang;CHEN Yi-fei;CAO Zhe;TIAN Wen-li;GUO Jun(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,Yunnan,China;Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]中国农业科学院蜜蜂研究所,北京100093

出　　处：《食品研究与开发》2021年第17期7-12,共6页Food Research and Development

基　　金：国家自然科学基金(31972087、31660695)。

摘　　要：为探究蜂王幼虫冻干粉对失眠小鼠的治疗作用,该研究通过腹腔注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA)来构建失眠小鼠模型,连续7 d对失眠小鼠分别灌胃低(0.1 g/kg)、中(1 g/kg)和高剂量(10 g/kg)的蜂王幼虫冻干粉。通过行为学观察和测定小鼠血清中5-羟色胺(5-hydroxytryptamine,5-HT)的含量来评价失眠模型和蜂王幼虫冻干粉的治疗及改善效果。结果表明,小鼠经PCPA腹腔注射后,与空白对照组相比血清中5-HT的相对体重含量都显著降低(P<0.05),且小鼠行为符合失眠模型特征;经过蜂王幼虫冻干粉灌胃后的失眠小鼠,与失眠模型组的小鼠相比血清中5-HT的含量显著升高(P<0.05),而与空白对照相比已无明显差异;另外,从行为学观察也发现失眠特征消失。An insomnia mice model was established through the intraperitoneal injection of p-chlorophenylalanine(PCPA)to explore the therapeutic effect of queen bee larvae on insomnia.Insomnia mice were given either a low(0.1 g/kg),medium(1 g/kg),or high dose(10 g/kg)of lyophilized queen bee larva powder for 7 days.The reliability of the insomnia model and the therapeutic effectiveness of queen bee larva powder were assessed by monitoring behavioral changes and 5-hydroxytryptamine(5-HT)content measurements in mice serum,respectively.The behavior of mice confirmed the effectiveness of the insomnia model and reliably recapitulated the characteristics of insomnia after intraperitoneal injection of PCPA.Furthermore,results showed that the relative bodyweight content of 5-HT in the serum of treated mice was significantly lower than in the insomnia-free control group(P<0.05).The content of 5-HT in the serum of queen bee larva powder-treated insomnia mice was significantly higher(P<0.05)than in the untreated insomnia mice.The insomnia-characteristic behavior disappeared in treated insomnia mice,unlike in the untreated insomnia mice group.Furthermore,there was no significant difference between queen bee larva powder-treated insomnia mice and the insomnia-free control group.

关 键 词：蜂王幼虫 对氯苯丙氨酸 失眠小鼠 5-羟色胺 睡眠障碍 

分 类 号：TS201.4[轻工技术与工程—食品科学]