金银花中肌醇的复合酶法提取及抗氧化活性评价  

Optimization of Enzymatic Extraction Conditions of Inositol from Flos Lonicerae and Its Antioxidant Activity

作　　者：马潇潇 田成 向福[1,2] 艾勇泉 MA Xiao-xiao;TIAN Cheng;XIANG Fu;AI Yong-quan(Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization,Huanggang Normal University,Huanggang 438000,Hubei,China;Hubei Zhongke Research Institute of Industrial Technology,Huanggang 438000,Hubei,China)

机构地区：[1]黄冈师范学院经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000 [2]湖北省中科产业技术研究院,湖北黄冈438000

出　　处：《食品研究与开发》2021年第17期101-106,共6页Food Research and Development

基　　金：湖北省高等学校优秀中青年科技创新团队计划项目(T201820)。

摘　　要：该试验以“罗田金银花”为材料,以肌醇提取率为指标,基于单因素和正交试验,建立纤维素酶和半纤维素酶协同提取金银花中肌醇的优化工艺。结果表明,在料液比1∶20(g/mL)和酶解温度60℃条件下,最佳提取工艺为纤维素酶和半纤维素酶质量比1∶1、复合酶添加量1.5%、酶解时间90 min,肌醇提取率可达(1.99±0.02)%,所得提取物具有抗氧化活性,其总抗氧化能力为相同质量浓度V_(C)的10.0%~12.8%。Based on a single factor analysis and an orthogonal test,the extraction of inositol from flos lonicerae using cellulase and hemicellulase was optimized,with extraction rate as an indicator.The optimum conditions were determined to be as follows:1∶1 mass ratio of cellulase and hemicellulase,addition of 1.5%complex enzymes,90 min duration of enzymolysis with 1:20 material to liquid ratio,and hydrolysis temperature of 60℃.The extraction rate of inositol was(1.99±0.02)%and its total antioxidant activity was 10.0%-12.8%of the same concentration of V_(C).

关 键 词：金银花 酶法提取 肌醇 正交试验 抗氧化 

分 类 号：R284.2[医药卫生—中药学]