山东省野生蛹虫草的驯化及杂交育种  

Domestication and Hybridisation of wild Cordyceps militaris in Shandong Province

作　　者：邱文旭 杨芳芳 贾玉萍 封金华 李健平 李维焕 盖宇鹏 程显好 QIU Wen-xu;YANG Fang-fang;JIA Yu-ping;FENG Jin-hua;LI Jian-ping;LI Wei-huan;GE Yu-peng;CHENG Xian-hao(Jinbao(shandong)food co.ltd,dongying 257300,china;shandong meiao biological engineering co.ltd,dongying 257300,china;agriculture school,ludong university,yantai 264025,china)

机构地区：[1]金孢(山东)食品有限公司,山东东营257300 [2]山东美奥生物工程有限公司,山东东营257300 [3]鲁东大学农学院,山东烟台264025

出　　处：《中国食用菌》2021年第7期15-20,共6页Edible Fungi of China

基　　金：山东省现代农业产业技术体系食用菌产业体系岗位专家项目(鲁农科技字(2016)18号)。

摘　　要：为选育性能优良的蛹虫草(Cordyceps militaris)生产菌株,对从山东省野外采集的36株蛹虫草标本进行了菌株分离和栽培试验,从中筛选得到1株高生物转化率菌株YC05和2株高虫草素含量菌株YC03、YC04。用钩悬法收集3株菌株子实体产生的子囊孢子,进行单孢杂交,从杂交子中初步选育出了15株出草菌株。通过栽培试验,比较了15株杂交菌株的生物转化率、虫草素、腺苷指标,其中YC4311、YC4315菌株的虫草素含量高于亲本,YC4308、YC4313、YC4521菌株的生物转化率高于亲本。因此,蛹虫草子囊孢子的单孢杂交是蛹虫草生产性育种的有效手段。In order to breed excellent Cordyceps militaris production strains,36 C.militaris specimens collected from the field in Shandong province were tested for strain isolation and cultivation.One strain with high bioconversion rate(YC05)and two strains with high cordycepin productivity(YC03,YC04)were screened.Ascospores collected from 3 different fruiting body with hook-hanging method were used for single ascospore hybrid,and 15 fruiting body producing strains were screened from the hybrids.Cultivating indicators(bioconversion rate,cordycepin and adenosine)were compared and analyzed based on cultivation test,2 strains(YC4311,YC4315)have higher cordycepin content than parents,and 3 strains(YC4308,YC4313,YC4521)have higher bioconversion rate than parents.Thus,single ascospore hybrid is a effective method for breeding C.militaris strains used for cultivation.

关 键 词：蛹虫草 子囊孢子 单孢杂交 虫草素 生物转化率 

分 类 号：S646.9[农业科学—蔬菜学]