Visualization analysis of lecithin in drugs based on electrochemiluminescent single gold microbeads  

作　　者：Gen Liu Pei-Long Wang Hui Gao 

机构地区：[1]College of Chemistry and Material Science,Huaibei Normal University,Huaibei,235000,Anhui,China [2]Henan Key Laboratory of Biomolecular Recognition and Sensing,Shangqiu Normal University,Shangqiu,476000,Henan,China [3]State Key Laboratory of Analytical Chemistry for Life Science,Nanjing University,Nanjing,210023,China

出　　处：《Journal of Pharmaceutical Analysis》2021年第4期515-522,共8页药物分析学报（英文版）

基　　金：supported by Anhui Provincial Natural Science Foundation(Grant Nos.2008085QB68 and 1808085QB29);Key Project of Provincial Natural Science Research Foundation of Anhui Universities(Grant Nos.KJ2018A0675 and KJ2018A0389);Foundation of State Key Laboratory of Analytical Chemistry for Life Science(Grant No.SKLACLS2003);Foundation of Henan Key Laboratory of Biomolecular Recognition and Sensing(Grant No.HKLBRSK1905)。

摘　　要：Fast and high-throughput determination of drugs is a key trend in clinical medicine.Single particles have increasingly been adopted in a variety of photoanalytical and electroanalytical applications,and microscopic analysis has been a hot topic in recent years,especially for electrochemiluminescence(ECL).This paper describes a simple ECL method based on single gold microbeads to image lecithin.Lecithin reacts to produce hydrogen peroxide under the successive enzymatic reaction of phospholipase D and choline oxidase.ECL was generated by the electrochemical reaction between a luminol analog and hydrogen peroxide,and ECL signals were imaged by a camera.Despite the heterogeneity of single gold microbeads,their luminescence obeyed statistical regularity.The average luminescence of 30 gold microbeads is correlated with the lecithin concentration,and thus,a visualization method for analyzing lecithin was established.Calibration curves were constructed for ECL intensity and lecithin concentration,achieving detection limits of 0.05 m M lecithin.This ECL imaging platform based on single gold microbeads exhibits outstanding advantages,such as high throughput,versatility and low cost,and holds great potential in disease diagnostics,environmental monitoring and food safety.

关 键 词：LECITHIN Single particle Visualization analysis ELECTROCHEMILUMINESCENCE 

分 类 号：R914[医药卫生—药物化学]