36份桑树种质资源桑叶1-脱氧野尻霉素含量与SSR标记遗传多样性的相关性分析  

Correlation of DNJ Content and Genetic Diversity of SSR Markers of 36Mulberry Germplasm Resources

作　　者：蔡翔[1,2,3] 胡桂萍 曹红妹[1,2,3] 张国彪 刘昭霞 胡丽春[1,2,3] 石旭平 Cai Xiang;Hu Guiping;CaoHongmei;Zhang Guobiao;Liu Zhaoxia;Hu Lichun;Shi Xuping(Jiangxi Sericulture and Tea Research Institute,Nanchang 330202,China;Economic Crops Research Institute of Jiangxi Province,Nanchang 33Q2Q2,China;Research Center for Silk and Mulberry Engineering&Technology in Jiang-xi,Nanchang 330202,China)

机构地区：[1]江西省蚕桑茶叶研究所,南昌330202 [2]江西省经济作物研究所,南昌330202 [3]江西省蚕桑工程技术研究中心,南昌330202

出　　处：《蚕业科学》2021年第3期293-299,共7页ACTA SERICOLOGICA SINICA

基　　金：江西省蚕桑产业技术体系项目(JXARS-23);。

摘　　要：1-脱氧野尻霉素(1-deoxynojirimycin,DNJ)在降血糖、降血脂、抗病毒和抗肿瘤中具有一定的药用价值,而桑树是已知植物中DNJ含量最高的物种。为了适应药用专用桑种质资源开发需求,对收集的36份桑树种质资源开展SSR标记遗传多样性和DNJ含量分析,探讨其内在关系,为高DNJ含量桑树种质资源的挖掘提供依据。结果表明,供试36份桑树种质资源的桑叶DNJ含量差异很大,平均值为1.2794 mg/g,变幅为1.03~1.61 mg/g,变异系数为0.1276;5对SSR引物的扩增产物在36份桑树种质资源间均存在多态性,共检测到32个基因型,等位基因数均值3.8,观测杂合度均值0.62,多态性信息量(PIC)0.61。基于SSR标记遗传距离的聚类分析显示供试36份桑树种质资源可分成3大类,而基于DNJ含量的聚类分析可将供试桑树种质资源分成2大类,2个聚类结果的比较分析显示同一类群桑树种质资源间DNJ含量相当。研究结果表明,桑树SSR标记遗传多样性与DNJ含量遗传多样性存在相似性。1-Deoxynojirimycin(DNJ)has certain medicinal values in lowering blood sugar,lowering blood fat,antiviral and anti-tumor,and mulberry has the highest DNJ content in known plants.To take full advantage of medicinal benefits of mulberry,the research was carried out on the internal relationships of the genetic diversity of SSR markers and DNJ content,so as to provide the basis for the exploitation and breeding of mulberry resources with high DNJ content.The results showed that the content of DNJ in the leaves of 36 mulberry germplasm resources varied greatly,with the mean value of 1.2794 mg/g,the amplitude of 1.03-1.61 mg/g,and the coefficient of variation of 0.1276.Polymorphism was found in by 5 pairs of SSR primers from 36 mulberry germplasm varieties,and a total of 32 genotypes were detected.The mean allele number was 3.8,the mean heterozygosity was 0.62,and the polymorphism information content(PIC)was 0.61.Cluster analysis based on the genetic distance of SSR markers showed that the 36 tested mulberry germplasm samples could be divided into 3 categories,and the analysis based on DNJ content showed that they could be divided into 2 categories.Comparison of the two cluster analyses displayed that the DNJ content in mulberry germplasm varieties of the same group was similar.The above results revealed that the genetic diversity of SSR markers was similar to the genetic diversity of DNJ content in mulberry leaf.

关 键 词：桑树 种质资源 1-脱氧野尻霉素 SSR标记 相关性 

分 类 号：S888.2[农业科学—特种经济动物饲养]