过表达miR-146a通过靶向SOX5抑制胃癌MKN-45细胞增殖和侵袭  

Over-expression of miR-146a inhibits proliferation and invasion of gastric cancer MKN-45 cells by targeting SOX5

作　　者：宋成飞[1] 孙明立[2] 李涛[1] 王舒[1] 杜健 王江[1] SONG Cheng-fei;SUN Ming-li;LI Tao;WANG Shu;DU Jian;WANG Jiang(Department of Orthopaedics,Fushun Genetal Hospital,Liaoning Healtrh Industry Group,Fushun 113008;Department of Pharmacology,China Medical University,Shenyang 110122,China)

机构地区：[1]辽宁健康产业集团抚顺矿务局总医院肝胆胰甲状腺微创外科,辽宁抚顺113008 [2]中国医科大学药理学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2021年第4期475-477,481,共4页Progress of Anatomical Sciences

基　　金：辽宁省教育厅青年育苗项目(GN2019033)。

摘　　要：目的探讨过表达miR-146a对胃癌MKN-45细胞增殖、侵袭的影响及可能机制。方法利用瞬时转染将miR-146a mimics(过表达miR-146a组)和miR-NC(阴性对照组)分别转染胃癌MKN-45细胞,建立过表达miR-146a胃癌MKN-45细胞系,采用MTT法检测转染后MKN-45细胞增殖能力,Transwell实验检测转染后MKN-45细胞侵袭能力。Western blot检测转染后MKN-45细胞中SOX5蛋白表达,通过网站Targetscan预测SOX5是否为miR-146a的靶基因,双荧光素酶实验进行验证。结果 miR-146a过表达抑制MKN-45细胞增殖、侵袭能力,miR-146a过表达使MKN-45细胞中SOX5蛋白表达下降,Targetscan网站分析及双荧光素酶实验证实SOX5为miR-146a的靶基因。结论过表达miR-146a可以抑制胃癌MKN-45细胞增殖及侵袭能力,其机制可能与下调SOX5的表达有关。Objective To investigate the effect and mechanism of over-expression of miR-146 a on the proliferation and invasion of gastric MKN-45 cells. Methods Instantaneous transfection of miR-146 a mimics and miRNC in gastric MKN-45 cells, the transfection efficiency was verified by real-time PCR. MTT was used to detect the proliferation, Transwell was used to detect the invasion, Western blot was used to detect the expression of SOX5 protein, and the Targetscan and double luciferase reporter gene experiment was used to verify whether SOX5 is the target gene of miR-146 a. Results Over-expression of miR-146 a could inhibit the proliferation and invasion of gastric MKN-45 cells, and the expression of SOX5 protein was decreased. Bioinformatics and dual luciferase reporter gene confirmed that SOX5 was the target gene of miR-146 a. Conclusion miR-146 a could inhibit the proliferation and invasion of gastric MKN-45 cells by targeting SOX5.

关 键 词：miR-146 MKN-45细胞 增殖 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]