普萘洛尔对血管瘤裸鼠模型血管生成素及Tie2的影响  

Effect of propranolol on angiopoietin and Tie2 in nude mice model with hemangioma

作　　者：沃贝贝 王大鹏 杨建民 Wo Beibei;Wang Dapeng;Yang Jianmin(Department of Plastic Surgery,980 Hospital of PLA Joint Logistics Support Force,Shijiazhuang 050000,China;Fengfeng General Hospital of Medical and Health Group,Handan 056200,China;Department of Plastic Surgery,Beijing Qinghua Changgeng Hospital,Beijing 102218,China)

机构地区：[1]解放军联勤保障部队第980医院整形外科,石家庄050000 [2]华北医疗健康集团峰峰总医院外科,河北邯郸056200 [3]北京清华长庚医院整形外科,北京102218

出　　处：《中华医学美学美容杂志》2021年第4期325-329,共5页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的探讨普萘洛尔对增殖期婴幼儿血管瘤裸鼠模型中血管生成素及受体的影响。方法2015年5月至2016年9月,解放军联勤保障部队第980医院整形外科手术切除增殖期血管瘤体组织,将其移植于50只雌性裸鼠背部,构建血管瘤裸鼠动物模型后分为普萘洛尔组和对照组,每组25只。普萘洛尔组每2天使用0.4 ml普萘洛尔灌胃,对照组每2天使用生理盐水灌胃。于7、14、21、28 d对血管瘤体进行HE染色,对瘤体形态进行观察,免疫组织化学分析、Western印迹法检测血管瘤组织中血管内皮生长因子(VEGF)、Ang1、Ang2、Tie2蛋白表达。结果28 d后,普萘洛尔组血管瘤瘤体呈消退期表现,血管瘤内皮细胞减少,细胞核染色变浅,管腔增大,血管间可见纤维结缔组织,VEGF、Ang2、Tie2表达较对照组分别降低47.1%、34.7%、37.5%(P<0.05),而Ang1表达增加,数量较对照组增多40.5%(P<0.05),与对照组相比差异有统计学意义(P<0.05)。结论普萘洛尔对裸鼠血管瘤生长的抑制作用,可能是通过促进Ang1表达,抑制VEGF、Ang2、Tie2表达得以实现。Objective To investigate the effect of propranolol on angiopoietin and its receptor in the nude mouse model of infantile hemangioma,so as to clarify the mechanism of propranolol in the treatment of hemangioma.Methods The proliferative hemangioma tissue from the 980 Hospital of PLA was transplanted into the back of 50 female nude mice during May 2015 to Sept.2016.After establishment of the hemangioma model,the nude mice were randomly divided into two groups,25 in each group.The experimental group was gavaged with 0.4 ml propranolol every 2 days,and the control group was gavaged with normal saline every 2 days.The mice were killed in batches on the 7th,14th,21st and 28th day,the tumor morphology was observed by HE staining.The expression of VEGF,Ang1,Ang2 and Tie2 protein in hemangiomas was detected by immunohistochemistry and Western blot.Results After 28 days,the hemangioma in propranolol group showed regression phase.The endothelial cells of hemangioma decreased,and the nuclear staining became shallow,the lumen enlarged,and fibrous connective tissue could be seen between the blood vessels.The expression of VEGF,Ang2 and Tie2 was lower than those in the control group(P<0.05),and the expression of VEGF,Ang2 and Tie2 was decreased by 47.1%,34.7%and 37.5%,respectively,while the expression of Ang1 was increased by 40.5%compared with the control group(P<0.05).Conclusions Propranolol may inhibit the growth of hemangioma in nude mice by promoting the expression of Ang1 and inhibiting the expression of VEGF,Ang2 and Tie2.

关 键 词：血管瘤 普萘洛尔 促血管生成素 血管内皮生长因子 TIE2受体 

分 类 号：R732.2[医药卫生—肿瘤]