肺腺癌移植瘤裸鼠模型侵袭组织中酪氨酸蛋白激酶受体A7、表皮生长因子受体和Ki67蛋白表达及意义  

Expression and significance of EphA7,EGFR and Ki67 proteins in invasion tissues of lung adenocarcinoma transplanted tumor in nude mice

作　　者：王徽 白玉勤 WANG Hui;BAI Yuqin(Jinzhou Medical University,Jinzhou 121001,China)

机构地区：[1]锦州医科大学,辽宁锦州121001 [2]赤峰学院附属医院神经内科,内蒙古赤峰024000 [3]赤峰学院第二附属医院赤峰市肿瘤医院病理科,内蒙古赤峰024000

出　　处：《陕西医学杂志》2021年第9期1060-1063,共4页Shaanxi Medical Journal

基　　金：内蒙古自治区高等学校科学研究项目(NJZZ18202)。

摘　　要：目的:探讨肺腺癌移植瘤裸鼠模型侵袭组织中酪氨酸蛋白激酶受体A7(EphA7)、表皮生长因子受体(EGFR)、Ki67蛋白表达及意义。方法:通过皮下接种肺腺癌A549细胞的方式建立肺腺癌移植瘤裸鼠模型,通过免疫组化染色观察肺腺癌移植瘤侵袭组织与非侵袭组织中EphA7、EGFR、Ki67蛋白表达情况并进行半定量分析。结果:肺腺癌移植瘤侵袭组织与非侵袭组织中EphA7蛋白表达均为阳性,EGFR蛋白表达均为阴性,阳性细胞表达率比较差异无统计学意义(均P>0.05)。肺腺癌移植瘤侵袭组织中Ki67蛋白表达均为阳性,非侵袭组织中Ki67蛋白表达为阴性或弱阳性,侵袭组织阳性细胞表达率高于非侵袭组织(P<0.05)。结论:EphA7、EGFR蛋白表达与肺腺癌侵袭进展无明显关系,Ki67蛋白表达可能会影响肺腺癌侵袭。Objective:To investigate the expression and significance of ephrin receptor A7(EphA7),epidermal growth factor receptor(EGFR)and Ki67 proteins in invasion tissues of lung adenocarcinoma transplanted tumor in nude mice.Methods:Nude mouse models of lung adenocarcinoma transplanted tumor were established by subcutaneous vaccination of lung adenocarcinoma A549 cells,and immunohistochemical staining was used to observe the expression of EphA7,EGFR,and Ki67 proteins in invasive and non-invasive tissues of lung adenocarcinoma transplanted tumor.Meanwhile,semi-quantitative analysis was conducted.Results:The expression of EphA7 protein was positive in invasive and non-invasive tissues of lung adenocarcinoma transplanted tumor,and the expression of EGFR protein was negative,and there was no significant difference in the expression rate of positive cells(all P>0.05).The expression of Ki67 protein was positive in invasive tissues,and was negative or weakly positive in non-invasive tissues,and the expression rate of positive cells in invasive tissues was higher than that in non-invasive tissues(P<0.05).Conclusion:The expression of EphA7 and EGFR proteins has no obvious relationship with the invasion and progression of lung adenocarcinoma,and the expression of Ki67 protein may affect the invasion of lung adenocarcinoma.

关 键 词：肺腺癌 裸鼠模型 肿瘤侵袭 酪氨酸蛋白激酶受体A7 表皮生长因子受体 KI67蛋白 

分 类 号：R734.2[医药卫生—肿瘤]