高侵袭性膀胱癌细胞中的Wnt7a和MMP-10表达及相关性分析  

Expression and Correlation Analysis of Wnt7a and MMP-10 in Highly Invasive Bladder Cancer Cells

作　　者：杨天[1] 吴明亮 张国海 YANG Tian;WU Mingliang;ZHANG Guohai(Department of Urology Surgery,Dazhou Integrated TCM&Western Medicine Hospital,Dazhou 635000,China)

机构地区：[1]达州市中西医结合医院泌尿外科,达州635000

出　　处：《宁夏医科大学学报》2021年第8期773-776,781,共5页Journal of Ningxia Medical University

摘　　要：目的探讨Wnt7a和MMP-10在高侵袭性膀胱癌细胞中的表达作用及两者之间的相关性。方法通过qRT-PCR和Western blot检测高侵袭膀胱癌细胞(5637 HUBC)和对照低侵袭性膀胱癌细胞(5637 NUBC)中Wnt7a和MMP-10的表达情况,通过Wnt7a siRNA转染HUBC细胞,检测转染后Wnt7a和MMP-10的表达情况,通过Millicell小室检测转染后5637 HUBC细胞的侵袭能力,收集膀胱癌(BC)组织标本和癌旁组织,通过qRT-PCR和Western blot检测两者表达情况,Pearson相关分析两者相关性。结果与对照5637 NUBC相比,5637 HUBC细胞中Wnt7a和MMP-10的mRNA表达水平增加,并且Wnt7a和MMP-10的蛋白质表达水平也增加(P均<0.01);转染两种不同的siRNA(siWnt7a-1和siWnt7a-2)和对照siRNA(siCTL)后,Wnt7a的mRNA表达降低,Wnt7a和MMP-10蛋白水平在功能丧失实验后均降低,经过Transwell实验发现,与对照组比较,敲除Wnt7a后,5637 HUBC的侵袭能力减低;BC组织和正常癌旁组织Wnt7a和MMP-10的表达差异明显(P<0.01),Wnt7a和MMP-10表达呈正相关(r=0.584,P=0.002)。结论Wnt7a及其下游基因MMP-10在BC细胞侵袭性方面发挥重要作用,Wnt7a及其相关的MMP-10基因位点有希望成为BC细胞侵袭和转移的药物靶点。Objective To detect the expression of Wnt7a and MMP-10 in highly invasive bladder cancer cells and to analyze its correlation.Methods The expression of Wnt7a and MMP-10 in bladder cancer cells with high invasion(5637 HUBC)and control low invasion(5637 NUBC)were detected by fluorescence quantitative PCR and Western blot.HUBC cells were transfected with Wnt7a siRNA to detect the expression of Wnt7a and MMP-10 after transfection.Millicell chamber was used to detect the invasion ability of HUBC cells after transfection.UBC tissue specimens and adjacent tissues were collected,and qRT-PCR and Western blot were used to detect the expression of the two proteins,and Pearson correlation was used to analyze the correlation between Wnt7a and MMP-10.Results Compared with the control 5637 NUBC,the expression levels of Wnt7a and MMP-10 mRNA and protein by highly invasive 5637 HUBC cells were significantly increased(P all<0.01).After transfected with two different siRNAs(siWnt7a-1 and siWnt7a-2)and control siRNA(siCTL),Wnt7a mRNA expression was significantly reduced,and Wnt7a and MMP-10 protein levels were significantly reduced after the loss of function experiment.The Transwell experiment found that compared with the control group,after knocking out Wnt7a,the invasion ability of 5637 HUBC was significantly reduced.The difference in expression of Wnt7a and MMP-10 between UBC tissues and normal tissues was very obvious(P<0.01),and the expression of Wnt7a and MMP-10 showed a positive correlation(r=0.584,P=0.002).Conclusion Wnt7a and its downstream gene MMP-10 play an important role in the aggressiveness of highly invasive UBC,so targeting Wnt7a and MMP-10 is promising as a new drug target for the treatment of aggressive bladder cancer.

关 键 词：Wnt7a 膀胱癌 侵袭 MMP-10 

分 类 号：R737.14[医药卫生—肿瘤]