A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis  

作　　者：Xishi Zhou Junna He Christos N.Velanis Yiwang Zhu Yuhan He Kai Tang Mingku Zhu Lisa Graser Erica de Leau Xingang Wang Lingrui Zhang W.Andy Tao Justin Goodrich Jian-Kang Zhu Cui-Jun Zhang 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,518124 China [2]College of Horticulture,China Agricultural University,Beijing,100193 China [3]Department of Horticulture and Landscape Architecture,Purdue University,West Lafayette,IN 47907 USA [4]Institute of Molecular Plant Science,School of Biological Sciences,University of Edinburgh,Daniel Rutherford Building,Max Born Crescent,Edinburgh,EH93BF United Kingdom [5]Shanghai Center for Plant Stress Biology and CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai,201602 China [6]Institute of Integrative Plant Biology,School of Life Sciences,Jiangsu Normal University,Xuzhou,221116 China [7]University of Applied Sciences Mannheim,Paul-Wittsack-Str.10,Mannheim,68163 Germany [8]Department of Biochemistry,Purdue University,West Lafayette,IN 47907 USA

出　　处：《Journal of Integrative Plant Biology》2021年第8期1462-1474,共13页植物学报（英文版）

基　　金：This work was supported by grants from the National Key Research and Development Program of China(2020YFE0202300);the Central Public-interest Scientific Institution Basal Research Fund,the BBSRC under the Grant Reference BB/P008569/1 to J.G.C.N.V.and E.dL.,and an Erasmus plus training award to L.G.

摘　　要：In eukaryotes,histone acetylation is a major modification on histone N-terminal tails that is tightly connected to transcriptional activation.HDA6 is a histone deacetylase involved in the transcriptional regulation of genes and transposable elements(TEs)in Arabidopsis thaliana.HDA6 has been shown to participate in several complexes in plants,including a conserved SIN3 complex.Here,we uncover a novel protein complex containing HDA6,several Harbinger transposon-derived proteins(HHP1,SANT1,SANT2,SANT3,and SANT4),and MBD domain-containing proteins(MBD1,MBD2,and MBD4).We show that mutations of all four SANT genes in the sant-null mutant cause increased expression of the flowering repressors FLC,MAF4,and MAF5,resulting in a late flowering phenotype.Transcriptome deep sequencing reveals that while the SANT proteins and HDA6 regulate the expression of largely overlapping sets of genes,TE silencing is unaffected in sant-null mutants.Our global histone H3 acetylation profiling shows that SANT proteins and HDA6 modulate gene expression through deacetylation.Collectively,our findings suggest that Harbinger transposon-derived SANT domain-containing proteins are required for histone deacetylation and flowering time control in plants.

关 键 词：DEACETYLATION FLOWERING Harbinger HDA6 histone acetylation protein complex SANT 

分 类 号：Q943.2[生物学—植物学]