Plant-based,adjuvant-free,potent multivalent vaccines for avian influenza virus via Lactococcus surface display  

作　　者：Shi-Jian Song Gyeong-Im Shin Jinyong Noh Jiho Lee Deok-Hwan Kim Gyeongryul Ryu Gyeongik Ahn Hyungmin Jeon Hai-Ping Diao Youngmin Park Min Gab Kim Woe-Yeon Kim Young-Jin Kim Eun-Ju Sohn Chang Seon Song Inhwan Hwang 

机构地区：[1]Department of Life Science,Pohang University of Science and Technology,Pohang,37673 Korea [2]College of Pharmacy and Research Institute of Pharmaceutical Science,Gyeongsang National University,Jinju,660-701 Korea [3]KCAV Inc.,Gwangjin-gu,05029 Korea [4]Avian Disease Laboratory,College of Veterinary Medicine,Konkuk University,Seoul,05029 Korea [5]Division of Applied Life Science(BK21 PLUS),Institute of Agriculture&Life Sciences,Gyeongsang National University,Jinju,52828 Korea [6]Bioapp,Inc.,Pohang Technopark Complex,Pohang,37668 Korea

出　　处：《Journal of Integrative Plant Biology》2021年第8期1505-1520,共16页植物学报（英文版）

基　　金：This work was supported by the Ministry of Trade,Industry and Energy(grant number,10063301);by the National Research Foundation grants funded by the Ministry of Science and Information Technology(No.2019R1A2B5B-03099982 and 2019R1A2C1087207),Korea.

摘　　要：Influenza epidemics frequently and unpredictably break out all over the world,and seriously affect the breeding industry and human activity.Inactivated and live attenuated viruses have been used as protective vaccines but exhibit high risks for biosafety.Subunit vaccines enjoy high biosafety and specificity but have a few weak points compared to inactivated virus or live attenuated virus vaccines,especially in low immunogenicity.In this study,we developed a new subunit vaccine platform for a potent,adjuvant-free,and multivalent vaccination.The ectodomains of hemagglutinins(HAs)of influenza viruses were expressed in plants as trimers(tHAs)to mimic their native forms.tHAs in plant extracts were directly used without purification for binding to inactivated Lactococcus(iLact)to produce iLact-tHAs,an antigen-carrying bacteria-like particle(BLP).tHAs BLP showed strong immune responses in mice and chickens without adjuvants.Moreover,simultaneous injection of two different antigens by two different formulas,t^(HAH5N6+H9N2) BLP or a combination of t^(HAH5N6) BLP and t^(HAH9N2) BLP,led to strong immune responses to both antigens.Based on these results,we propose combinations of plant-based antigen production and BLP-based delivery as a highly potent and cost-effective platform for multivalent vaccination for subunit vaccines.

关 键 词：bacteria-like particle(BLP) H5N6 H9N2 Lactococcus lactis multi-valent vaccine plant-based vaccine trimeric HA 

分 类 号：R392-33[医药卫生—免疫学]