响应面法优化异养培养条件提高链带藻Z8油脂产量的研究  

Enhancement of Lipid Production in Desmodesmus intermedius Z_(8) by Optimization of Heterotrophic Culture Conditions using Response Surface Method

作　　者：李敏 白耘榧 黎秋玲 李志 周智友 李汉广[1] LI Min;BAI Yunfei;LI Qiuling;LI Zhi;ZHOU Zhiyou;LI Hanguang(Institute of Applied Microbiology,Jiangxi Agricultural University,Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources,College of Biological Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学生物科学与工程学院/江西农业大学应用微生物研究所/江西省菌物资源保护与利用重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2021年第4期910-918,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(21466014);江西省自然科学基金项目(20202BABL203042);江西省研究生创新专项资金项目(YC2018-S198);江西农业大学博士启动基金项目(9232305387)。

摘　　要：【目的】微藻油脂含量过低是阻碍微藻生物柴油工业化的主要障碍之一,研究拟通过单因子优化以及响应面法优化链带藻Z8(Desmodesmusintermedius Z8)异养培养条件,从而提高其油脂产量。【方法】通过单因素试验探究不同浓度条件下的葡萄糖、硫酸镁、柠檬酸、磷酸氢二钾、硝酸钠及氯化钙等对藻种Z8生物量和总脂量的影响;在此基础上利用P-B试验从6个考察因素中筛选出显著影响因素,最后通过CCD试验来确定最佳工艺条件。【结果】通过P-B试验筛选出4个显著影响因素,分别为:葡萄糖、硫酸镁、柠檬酸、磷酸氢二钾;通过CCD试验获得了最佳工艺条件,具体如下:硫酸镁质量浓度200 mg/L、磷酸氢二钾质量浓度0.07 g/L、葡萄糖质量浓度18 g/L、柠檬酸质量浓度9 mg/L、硝酸钠质量浓度1.5 g/L及氯化钙质量浓度0.04 g/L。在此条件下藻株Z8的生物量可达到7.7 g/L,与未优化前的生物量5.47 g/L相比提高了40.77%,总脂量可达3.17 g/L,与优化前的总脂量2.47 g/L相比提高了128%。【结论】经响应面法优化后的链带藻Z8生物量和总脂量均有较大提高,研究所采用的递进式优化方法可为提高微藻的油脂产量及其它发酵工艺的提升提供有益的参考。[Objective]With the increasing depletion of fossil energy,biomass energy as an alternative energy source has become more concerned.Biodiesel is considered to be one of the best alternative energy sources to solve the problem of limited fossil fuel reserves due to its extremely high similarity to fossil diesel in terms of energy density and combustion performance.Currently,most biodiesel is produced worldwide from oil crops or animal oil materials.When the edible oil crops were used as raw materials for the production of biodiesel,it would compete with the food market,and then lead to food shortages.Microalgae can grow 10-50 folds faster than terrestrial plants.Nutritional composition such as lipids,carbohydrates and proteins of microalgae is 3 folds more than those derived from plants and fish.They can be cultivated on non-arable land without seasonal limitation.At the same time,the harvesting could be in a daily basis.Therefore,microalgae have been widely regarded as one of the most promising raw materials for biofuels.Nevertheless,one of the main factors hindering the industrialization of microalgae biodiesel is its low production of microalgae lipid.In this study,to enhance the lipid production,the total lipid production of the microalgae strain Z8 was used as the evaluation index,microalgae heterotrophic medium conditions were further optimized.Specifically,the most significant process parameters such as glucose,magnesium sulfate,sodium nitrate,calcium chloride,dipotassium hydrogen phosphate and citric acid were selected for the optimization through using the method of one-variable-at-a time and response surface methodology.[Method]The effects of different ingredient concentrations in the medium,such as glucose,magnesium sulfate,citric acid,dipotassium hydrogen phosphate,sodium nitrate,and calcium chloride,on the biomass and lipid production were investigated by using the method of single factor experiment.After completing the above steps,the main influencing factors were found by the Plackett-Burman(P-B)scre

关 键 词：微藻 总脂量 生物质能源 响应面法 

分 类 号：TQ92[轻工技术与工程—发酵工程]