Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity  

作　　者：Xiangqi Fan Haiyan Hu Daiwen Chen Bing Yu Jun He Jie Yu Junqiu Luo Erik Eckhardt Yuheng Luo Jianping Wang Hui Yan Xiangbing Mao 

机构地区：[1]Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Key laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs,Key laboratory of Animal Disease-resistant Nutrition of Sichuan Province,Institute of Animal Nutrition,Sichuan Agricultural University,No.211,Gongpinghuimin Road,Wenjiang District,Chengdu 611130,Sichuan Province,People’s Republic of China [2]Adisseo SAS,Center of Excellence and Research in Nutrition,03600 Malicorne,France

出　　处：《Journal of Animal Science and Biotechnology》2021年第3期1125-1137,共13页畜牧与生物技术杂志（英文版）

基　　金：This study was financially supported by the grant from the earmarked fund for the China Agriculture Research System(CARS-35);the fund from the Project of SAU-Adisseo Centre of Research on Nutrition and Health;the fund from Agricultural Science and Technology Achievement Transformation Fund Project of Sichuan Province(2016NYZ0052 and 20NZZH0057).

摘　　要：Background:Lentinan(LNT)may regulate many important physiological functions of human and animals.This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)-challenged weaned pigs.Methods:Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d(n=14).RV infection was executed on d 15.After extracting polysaccharides from LNT product,its major monosaccharides were analyzed.Then,LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells.Results:Dietary LNT supplementation supported normal function of piglets even when infected with RV,as reflected by reduced growth performance loss and diarrhea prevalence,and maintained gut immunity(P<0.05).The polysaccharide was isolated from LNT product,which molecular weight was 5303 Da,and major monosaccharides included glucose,arabinose and galactose.In RV-infected IPEC-J2 cells,this polysaccharide significantly increased cell viability(P<0.05),and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides(P<0.05).Conclusion:Those results suggest that LNT administration increases the piglets’resistance to RV-induced stress,likely by supporting intestinal immunity.

关 键 词：Gut immunity IPEC-J2 cells LENTINAN ROTAVIRUS Weaned pigs 

分 类 号：S858.28[农业科学—临床兽医学]