GSH对铅胁迫下多年生黑麦草生长及光合生理的影响  

Effects of reduced glutathione on the growth and photosynthesis of perennial ryegrass under lead stress

作　　者：赵利清[1,2] 彭向永[3] 刘俊祥[1] 毛金梅[4] 孙振元[1] ZHAO Li-qing;PENG Xiang-yong;LIU Jun-xiang;MAO Jin-mei;SUN Zhen-yuan(Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;The Open University of China,Beijing 100039,China;College of Life Science Qufu Normal University,Qufu 273165,China;Economic Forest Research Institute,Xinjiang Academy of Forestry,Urumqi 830063,China)

机构地区：[1]国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091 [2]国家开放大学,北京100039 [3]曲阜师范大学生命科学学院,山东曲阜273165 [4]新疆林业科学院经济林研究所,新疆乌鲁木齐830063

出　　处：《草业学报》2021年第9期97-104,共8页Acta Prataculturae Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2018ZB002)资助。

摘　　要：为了明确谷胱甘肽(GSH)对多年生黑麦草铅(Pb)毒害的缓解作用及其生理机制,以12周龄多年生黑麦草‘卡特’幼苗为试验材料,设置4个试验处理:1)根部置1/2 Hoagland营养液,叶面喷蒸馏水50 mL(CK);2)根部置含0.75 mmol·L^(-1)Pb(NO_(3))_(2)的1/2 Hoagland营养液,叶面喷蒸馏水50 mL(Pb);3)根部置含0.75 mmol·L^(-1)Pb(NO_(3))_(2)的1/2 Hoagland营养液,叶面先喷25 mL蒸馏水,待吸收后再喷施25 mL 10 mmol·L^(-1)GSH(Pb+GSH);4)根部置含0.75 mmol·L^(-1)Pb(NO_(3))_(2)的1/2 Hoagland营养液,叶面先喷25 mL蒸馏水,待吸收后再喷施25 mL 1 mmol·L^(-1)丁硫氨酸-亚砜亚胺(BSO)(Pb+BSO),研究GSH对多年生黑麦草生长及光合作用的影响。结果表明:叶面喷施GSH显著增加了幼苗茎叶长、根长、分蘖数、生物量、叶绿素含量和光合参数,也增加了叶绿素荧光动力学参数。叶面喷施BSO后,根长、分蘖数、生物量、类胡萝卜素含量和光合参数均降低。综上所述,Pb胁迫影响了光合作用,最终抑制了植物的生长。叶面喷施10 mmol·L^(-1)GSH能够缓解Pb对多年生黑麦草生长和光合作用的胁迫,提高植物的抗性。相反,叶面喷施1 mmol·L^(-1)BSO能够加剧Pb对植物的胁迫。The aim of this study was to explore the mitigative effects of reduced glutathione(GSH)on the growth and photosynthesis of perennial ryegrass(Lolium perenne) under lead stress. Twelve-week old seedlings of the perennial ryegrass cultivar‘cuttle’were used as the experimental material. In the control(CK),leaves were sprayed with 50 mL distilled water,and roots were supplied with 1/2-strength modified Hoagland’s nutrient solution. In the other three experimental treatments,the roots were supplied with 1/2-strength modified Hoagland’s nutrient solution containing 0. 75 mmol·L^(-1) Pb(NO_(3))_(2),and the leaves were sprayed with 50 mL distilled water(Pb treatment);with 25 mL distilled water followed by 25 mL 10 mmol·L^(-1) GSH(Pb+GSH treatment);or with 25 mL distilled water followed by 25 mL 1 mmol·L^(-1) L-buthionine-sulfoximine(BSO)(Pb+BSO treatment). The results showed that shoot length, root length, tiller number, biomass, chlorophyll content, photosynthetic parameters,and the kinetic parameters of chlorophyll fluorescence were significantly higher in the Pb+GSH treatment than in Pb treatment. In contrast,the values for root length,tiller number,biomass,carotenoid content,and photosynthetic parameters were lower in the Pb+BSO treatment than in Pb treatment. Together,our results show that spraying ryegrass leaves with 10 mmol·L^(-1) GSH can effectively alleviate the inhibitory effects of Pb stress and improve the Pb tolerance of perennial ryegrass,whereas spraying with BSO at 1 mmol·L^(-1) exacerbates the inhibitory effects of Pb stress.

关 键 词：多年生黑麦草 谷胱甘肽 生长 光合作用 铅 

分 类 号：S543.6[农业科学—作物学] X173[环境科学与工程—环境科学]