长链非编码RNA BCYRN1对SKOV3细胞恶性生物学行为的影响  

Effect of long chain noncoding RNA BCYRN1 on malignant biological behavior of SKOV3 cells

作　　者：李喜梅 林丽 毛郁蕾 陈新宵 冯泽蛟 廖海红[2] LI Xi-mei;LIN Li;MAO Yu-lei;CHEN Xin-xiao;FENG Ze-jiao;LIAO Hai-hong(Department of Obstetrics and Gynecology,Wenzhou Central Hospital,Wenzhou,Zhejiang 325000,China;不详)

机构地区：[1]温州市中心医院妇产科,浙江温州325000 [2]湖州市中心医院妇产科,浙江湖州313003

出　　处：《中华全科医学》2021年第10期1658-1661,1728,共5页Chinese Journal of General Practice

基　　金：浙江省医药卫生科技计划项目(2019RC285)。

摘　　要：目的探讨长链非编码RNA brain cytoplasmic RNA 1在卵巢癌组织中的表达及对SKOV3细胞恶性生物学行为的影响。方法选取温州市中心医院2017年3月-2019年3月收治的32例卵巢癌患者癌组织及其相应的癌旁组织标本,正常卵巢上皮细胞系IOSE和卵巢癌细胞系(SKOV3、HO8910、A2780)购自美国ATCC;癌组织和癌细胞中BCYRN1的水平采用实时荧光定量法(qRT-PCR)检测;采用BCYRN1高表达质粒和低表达质粒及其相应的对照物转染SKOV3细胞,分别构建BCYRN1高表达和低表达的细胞系;采用BrdU法检测细胞的增殖;采用划痕愈合实验检测细胞的迁移;采用Transwell法检测细胞的侵袭。结果 BCYRN1在卵巢癌组织中的表达(5.64±1.02)高于在癌旁组织中的表达(2.12±0.65);BCYRN1在卵巢癌细胞中的水平为SKOV3(2.25±0.78)、HO8910(2.85±0.78)、A27809(3.12±1.01),显著高于IOSE(1.01±0.12);BCYRN1高表达能够促进SKOV3细胞的增殖、迁移和侵袭。结论 BCYRN1在卵巢癌中表达上调,其作为促癌基因促进卵巢癌的进展。Objective To investigate the expression of long chain non-coding RNA brain cytoplasmic RNA-1(BCYRN1) in ovarian cancer and its effect on the malignant biological behaviour of SKOV3 cells. Methods A total of 32 ovarian cancer tissues and their corresponding adjacent tissues treated in Wenzhou Central Hospital from March 2017 to March 2019 were selected. Normal ovarian epithelial cell lines(IOSE) and ovarian cancer cell lines(SKOV3, HO8910, A2780) were purchased from ATCC. The level of BCYRN1 was detected by real-time PCR. SKOV3 cells were transfected with BCYRN1 high-expression plasmid and low-expression plasmid and their corresponding control substances, and cell lines with high and low expression of BCYRN1 were constructed. BrdU assay was used to detect cell proliferation, scratch healing test was used to detect cell migration, and Transwell assay was used to detect cell invasion. Results The expression of BCYRN1 in ovarian cancer tissues(5.64±1.02) was higher than that in adjacent tissues(2.12±0.65), and the levels of BCYRN1 in SKOV3, HO8910 and A27809 cells were(2.25±0.78),(2.85±0.78) and(3.12±1.01), respectively, which were significantly higher than those of IOSE(1.01±0.12). The high expression of BCYRN1 promoted the proliferation, migration and invasion of SKOV3 cells. Conclusion The expression of BCYRN1 is up-regulated in ovarian cancer, and it can be used as an oncogene to promote the progression of ovarian cancer.

关 键 词：LncRNA brain cytoplasmic RNA 1 卵巢癌 SKOV3细胞株 增殖 转移 

分 类 号：R737.31[医药卫生—肿瘤] R730.231[医药卫生—临床医学]