PPAR-γ对人胰腺癌BxPc-3细胞增殖的影响  

Effect of PPAR-γon proliferation of human pancreatic cancer BxPc-3 cells

作　　者：李博[1] 施景龙 邱厚匡[1] 许鸣[1] Li Bo;Shi Jinglong;Qiu Houkuang;Xu Ming(Department of Gastroenterology,Guangdong Second Provincial General Hospital,Guangzhou 510317,China)

机构地区：[1]广东省第二人民医院消化内科,广州510317 [2]广州市第十二人民医院普通外科,广州510620

出　　处：《新医学》2021年第9期677-679,共3页Journal of New Medicine

基　　金：广东省医学科研基金(A2019279);。

摘　　要：目的通过促进人胰腺癌BxPc-3细胞中过氧化物酶体增殖物激活受体-γ(PPAR-γ)的表达,分析其对BxPc-3细胞增殖的影响。方法培养BxPc-3细胞,分别给予终浓度为5、10、20、40μmol/L的PPAR-γ激动剂罗格列酮处理细胞,蛋白免疫印迹法检测PPAR-γ蛋白的表达,四甲基偶氮唑蓝(MTT)法观察细胞增殖活性。结果BxPc-3细胞经5、10μmol/L罗格列酮处理后其PPAR-γ表达与对照组比较差异均无统计学意义(P均>0.05),经20、40μmol/L罗格列酮处理后PPAR-γ表达升高,与对照组比较差异有统计学意义(P均<0.05),且40μmol/L罗格列酮组PPAR-γ表达较20μmol/L罗格列酮组升高(P<0.05);BxPc-3细胞给予浓度为5、10μmol/L罗格列酮处理后BxPc-3细胞增殖活性分别为0.60±0.07、0.57±0.06,与对照组的0.62±0.09比较差异均无统计学意义(P均>0.05);经40μmol/L罗格列酮处理后BxPc-3细胞增殖活性为0.30±0.02,低于20μmol/L罗格列酮的0.45±0.03,且2组BxPc-3细胞增殖活性均低于对照组、5μmol/L罗格列酮组和10μmol/L罗格列酮组(P均<0.05)。结论促进PPAR-γ表达可以有效抑制人胰腺癌BxPc-3细胞增殖。Objective To evaluate the effect of promoting the expression of peroxisome proliferator-activated receptor-γ(PPAR-γ)on the proliferation of human pancreatic cancer BxPc-3 cells.Methods BxPc-3 cells were cultured and pretreated with PPAR-γagonist rosiglitazone with a final concentration of 5,10,20 and 40μmol/L,respectively.Western blot was used to detect the expression of PPAR-γprotein.MMT assay was employed to observe the cell proliferation.Results There was no statistical significance in the PPAR-γexpression levels between BxPc-3 cells treated with 5 and 10μmol/L rosiglitazone and control cells(both P>0.05).The expression levels of PPAR-γwere gradually up-regulated after treatment with 20 and 40μmol/L rosiglitazone,which had statistical significance compared with that in the control group(both P<0.05).The expression level of PPAR-γin the 40μmol/L rosiglitazone group was significantly higher than that in the 20μmol/L rosiglitazone group(P<0.05).The proliferative activity of BxPc-3 cells treated with 5 and 10μmol/L rosiglitazone was 0.60±0.07 and 0.57±0.06,which had no statistical significance compared with that in the control group(both P>0.05).The proliferative activity of BxPc-3 cells treated with 40μmol/L rosiglitazone was 0.30±0.02,significantly lower than those in the 20μmol/L rosiglitazone group(0.45±0.03),control group,5 and 10μmol/L rosiglitazone groups(all P<0.05).Conclusion Promoting PPAR-γexpression can effectively inhibit the proliferation of human pancreatic cancer BXPC-3 cells.

关 键 词：过氧化物酶体增殖物激活受体-Γ 胰腺癌 细胞增殖 

分 类 号：R735.9[医药卫生—肿瘤]