耐盐碱细菌筛选、鉴定及其降碱机理探究  

Study on Isolation and Identification of Salin-tolerant Bacteria and Its Degradation Characteristics

作　　者：宋建 赵尚昆 李方方[2] 王洋洋[2,3] SONG Jian;ZHAO Shang-kun;LI Fang-fang;WANG Yang-yang(Kaifeng Ecological Environment Monitoring Center of Henan Province,Kaifeng 475000,Henan,China;College of Environment and Planning,Henan University,Kaifeng 475000,Henan,China;National Demonstration Center for Environmental and Planning,Henan University,Kaifeng 475004,Henan,China)

机构地区：[1]河南省开封生态环境监测中心,河南开封475000 [2]河南大学环境与规划学院,河南开封475000 [3]河南大学环境与规划国家级教学示范中心,河南开封475004

出　　处：《有色金属（冶炼部分）》2021年第9期48-53,103,共7页Nonferrous Metals（Extractive Metallurgy)

基　　金：国家自然科学基金资助项目(51704093);中国博士后科学基金资助项目(2020M682284);河南省科技发展计划项目(212102310503)。

摘　　要：为解决高盐碱性赤泥高pH对赤泥堆场生态恢复的限制,从赤泥堆场中筛选出一株可高效降低赤泥pH的耐盐碱细菌菌株ZH1。研究显示,ZH1通过产酸的方式降低培养液pH,其最佳产酸培养基配比为葡萄糖8g/L、酵母膏3g/L、磷酸二氢钾0.3g/L、氯化镁0.3g/L。振荡培养时ZH1产生草酸含量最多,其次是α-酮戊二酸和乙酸、酒石酸;静置培养时ZH1产生草酸含量最多,其次是乳酸和酒石酸,但酒石酸都只在弱酸或中性条件下产生。两种培养方式最终均将培养液的pH降至6.0左右,振荡培养时所需时间较短(约48h),比静置培养快72h左右。通过对pH和有机酸之间的相关性检验发现,振荡培养时pH的下降主要是由草酸和α-酮戊二酸引起的,而静置培养时是由草酸、乳酸和酒石酸共同引起的。Red mud is a kind of dangerous solid waste produced by alumina industry and its ecological restoration is a difficult problem.To address limitation of high pH value of high salinity red mud on ecological restoration of red mud yard,a salt-tolerant bacteria strain ZH1 was screened from red mud yard,which could efficiently reduce pH value of red mud and was identified as Bacillus sp.by 16S rDNA analysis.The results show that ZH1 reduces pH value of red mud by producing acid,and its optimal acidproducing medium includes glucose of 8 g/L,yeast extract of 3 g/L,monopotassium phosphate of 0.3 g/L and magnesium chloride of 0.3 g/L.Content of oxalic acid produced by ZH1 is the highest,followed byα-ketoglutaric acid,acetic acid and tartaric acid in shaking culture;while content of oxalic acid produced by ZH1 is the highest,followed by lactate and tartaric acid in static culture,but tartaric acid is only produced under weakly acidic or neutral conditions.pH value of culture medium reduces to about 6.0,but the time required for shaking culture is shorter(about 48 h),which is about 72 hfaster than that of static culture.Through correlation test between pH value and organic acid,it is found that drop of pH in shaking culture is mainly caused by oxalic acid andα-ketoglutaric acid,while in static cultivation,it is mainly caused together by oxalic acid,lactic acid and tartaric acid.

关 键 词：赤泥 耐盐碱细菌 正交试验 机理 有机酸 

分 类 号：TF821[冶金工程—有色金属冶金]