青檀内生真菌高抗氧化活性菌株筛选及其培养条件优化  

Screening of High Antioxidant Activity Strains of Endophytic Fungi from Pteroceltis tatarinowii and Optimization of Culture Conditions

作　　者：柴新义 李梦宇 于士军 孙星 罗侠 张微微 胡文龙 CHAI Xinyi;LI Mengyu;YU Shijun;SUN Xing;LUO Xia;ZHANG Weiwei;HU Wenlong(School of Biological and Food Engineering,Chuzhou University,Chuzhou,Anhui 239000;Fengsheng Edible Fungus Production Cooperation,Daliu Town,Nanqiao District,Chuzhou,Anhui 239000)

机构地区：[1]滁州学院生物与食品工程学院,安徽滁州239000 [2]滁州南谯区大柳镇凤胜食用菌生产合作社,安徽滁州239000

出　　处：《武夷学院学报》2021年第6期23-28,共6页Journal of Wuyi University

基　　金：国家星火计划项目(2015GA710033);安徽省教育厅自然科学重点资助项目(KJ2018A0430)。

摘　　要：为合理开发和科学利用青檀植物内生真菌资源,以发酵粗提液的二苯代苦味肼基自由基(DPPH)清除率作为检测指标,筛选青檀内生真菌高抗氧化活性菌株,通过单因素和正交试验进行培养条件的优化研究。结果表明:在供试的30株青檀内生真菌中具有抗氧化活性的菌株有3株,占10%。高抗氧化活性菌株的最适发酵培养条件是:葡萄糖20 g/L、(NH_(4))_(2)SO_(4)2 g/L、K_(2)HPO_(4)3.2 g/L、CuSO_(4)6.0×10^(-3) g/L、MgSO_(4)0.2 g/L、最适装液量80 mL/250 mL、pH值7.0、温度27℃、培养时间8 d。In order to rationally develop and utilize the endophytic fungi resources of Pteroceltis tatarinowii,DPPH clearance rate was used as an indicator to screen high oxidation resistant strains and optimized culture by single factor and orthogonal experiments.The results showed that 30 strains of endophytic fungi were selected for determination of antioxidant activity,and 3 strains with antioxidant activity accounted for 10%.The optimal fermentation conditions were:glucose 20 g/L,(NH_(4))_(2)SO_(4)2 g/L,K_(2)HPO_(4)3.2 g/L,CuSO_(4)6×10^(-3) g/L,MgSO_(4)0.2 g/L,optimum liquid volume 80 mL/250 mL,pH 7.0,temperature 27℃,culture time 8 d.

关 键 词：青檀 内生真菌 高抗氧化活性 菌株筛选 培养条件优化 

分 类 号：Q939.5[生物学—微生物学]