中药翻白草黄酮类化学成分及其降糖活性研究  

Study on the Chemical Constituents of Flavonoids from Potentilla Discolor Bge.and Their Hypoglycemic Activity

作　　者：高颖 卢寅飞 张一笑 庞家乐 张春平 GAO Ying;LU Yin-fei;ZHANG Yi-xiao;PANG Jia-le;ZHANG Chun-ping(School of Pharmacy,Jiangsu Key Laboratory New Drug Research and Clinical Pharmacy,Xuzhou Medical University,Jiangsu Xuzhou 221004,China)

机构地区：[1]徐州医科大学药学院,江苏省新药研究与临床药学重点实验室,江苏徐州221004

出　　处：《广州化工》2021年第17期103-106,151,共5页GuangZhou Chemical Industry

基　　金：国家自然科学基金资助项目(31300222);江苏省自然科学基金资助项目(BK20181154)。

摘　　要：以翻白草为材料,探究黄酮类化学成分及其降糖活性降糖活性。采用系统溶剂法、柱色谱分离法对翻白草的化学成分进行分离,并鉴定化合物结构。基于HepG2细胞,筛选出具有降糖活性的化合物;采用胰岛素抵抗模型,筛选具有改善胰岛素抵抗的化合物。从翻白草乙醇提取物中分离得到13个化合物,分别鉴定为芹菜素(1)、木犀草素(2)、山柰酚(3)、山柰酚-3-O-β-D-吡喃葡萄糖苷(4)、山柰酚-3-O-β-D-葡萄糖醛酸苷(5)、山柰酚-3-O-β-D-半乳糖苷(6)、山柰酚-3-O-ɑ-L-阿拉伯糖苷(7)、槲皮苷(8)、槲皮素-3-O-β-D-吡喃葡萄糖苷(9)、槲皮素-7-O-β-D-吡喃葡萄糖苷(10)、槲皮素-3-O-β-D-葡萄糖醛酸苷(11)、异鼠李素(12)、异鼠李素-3-O-β-D-葡萄糖醛酸苷(13)。正常HepG2细胞实验中,化合物3、4、11、12、13组葡萄糖含量显著下降(P<0.05);胰岛素耐受细胞实验中,化合物3、9、11、13组葡萄糖含量显著下降(P<0.05),化合物3、13组的PPARγ蛋白表达显著上升(P<0.05)。化合物3、4、11、12、13能够促进正常的HepG2细胞葡萄糖消耗,化合物3、9、11、13能够改善胰岛素抵抗的HepG2细胞葡萄糖消耗。Study on the chemical constituents and hypoglycemic activity of flavonoids from Potentilla discolor bge..The system solvent method and column chromatography were used to separate the chemical components of Potentilla discolor bge.and identify the compound structure.Based on HepG2 cells,compounds with hypoglycemic activity are screened;insulin resistance models are used to screen compounds with improved insulin resistance,and the expression of PPARγprotein is detected.Thirteen compounds were isolated from the ethanol extract of potentilla discolor bunge chinensis,.They were identified as apigenin(1),luteolin(2),kaempferol(3),kaempferol-3-O-β-D-glucopyranoside(4),kaempferol-3-O-β-D-glucuronide(5),kaempferol-3-O-β-D-galactoside(6),kaempferol-3-O-α-L-arabinoside(7),quercitrin(8),quercetin-3-O-β-D-glucopyranoside(9),quercetin-7-O-β-D-glucopyranoside(10),quercetin-3-O-β-D-glucuronide(11),isorhamnetin(12),isorhamnetin-3-O-β-D-glucuronide(13).In the normal HepG2 cell experiment,the glucose content of the compound 3,4,11,12 and 13 group decreased significantly(P<0.05).In the insulin resistance cell experiment,the glucose content of the compound 3,9,11 and 13 group decreased significantly(P<0.05),the expression of PPARγprotein in groups 3 and 13 increased significantly(P<0.05).Compounds 3,4,11,12 and 13 can promote normal HepG2 cell glucose consumption,compounds 3,9,11 and 13 can improve insulin resistance in HepG2 cell glucose consumption and compounds 3 and 13 can promote PPARγprotein expression.

关 键 词：翻白草 黄酮 降糖活性 PPARΓ 

分 类 号：R284.1[医药卫生—中药学]