Phage displayed domain antibody mimics for pyrethroid and its application in immunoassay  

作　　者：Yanyan Zhao Ying Liang Yuan Liu Sicong Tu Shengming Zhao Xianjin Liu Kang Tu 

机构地区：[1]School of Food Science and technology,Henan Institute of Science and Technology,Xinxiang 453003,China [2]Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base,Key Laboratory of Control Technology and Standard for Agri-product Safety and Quality,Ministry of Agriculture and Rural Affairs,Institute of Agricultural Product Quality Safety and Nutrition Research,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China [3]College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China [4]School of Medical Sciences,University of New South Wales,Sydney,NSW 2052,Australia

出　　处：《International Journal of Agricultural and Biological Engineering》2020年第2期235-240,共6页国际农业与生物工程学报（英文）

基　　金：This work was supported by Key Science and Technology Project in Henan Province(202102110061,202102110060,182102110423);High Level Talent Research Project of Henan Institute of Science and Technology(2016018,2016019).

摘　　要：Anti-idiotypic antibodies which can mimic antigens have many potential applications in the immunoassay.This research used the monoclonal antibody of the conserved chemical region of pyrethroids,3-phenoxybenzoic acid(PBA),and the domain antibody library to develop an environmentally-friendly immunoassay for the detection of pyrethroids.The domain antibodies(A8,B8,and C6)which bound to anti-PBA monoclonal antibody(MAb)were isolated from a naive phage display human domain antibody library.This domain antibody is cloneable,pyrethroid-free,and applicable as a competitive mimetic antigen in the immunoassay.The best immunoassay was achieved using A8,resulting in IC50 of 0.714μg/mL for PBA,1.775μg/mL for Cypermethrin,1.624μg/mL forβ-cypermethrin,3.675μg/mL for Fenvalerate,and 4.895μg/mL for Flucythrinate.This way of selecting anti-idiotypic antibodies to detect pyrethroids could provide potential applications in developing immunoassays for identifying various chemical contaminants in food.

关 键 词：domain antibody enzyme-linked immunosorbent assay phenoxybenzoic acid PYRETHROID 

分 类 号：R36[医药卫生—病理学]