In-situ and one-step preparation of protein film in capillary column for open tubular capillary electrochromatography enantioseparation  

作　　者：Ling Li Xuqi Xue Huige Zhang Wenjuan Lv Shengda Qi Hongying Du Anne Manyande Hongli Chen 

机构地区：[1]State Key Laboratory of Applied Organic Chemistry,College of Chemistry and Chemical Engineering Lanzhou University [2]Key Laboratory of Environment Correlative Dietology,Ministry of Education,College of Food Science and Technology,Huazhong Agricultural University [3]School of Human and Social Sciences,University of West London

出　　处：《Chinese Chemical Letters》2021年第7期2139-2142,共4页中国化学快报（英文版）

基　　金：the financial support from the National Natural Science Foundation of China(No.21874060);the Special Funding for Open and Shared Large-Scale Instruments and Equipments of Lanzhou University(No.LZU-GXJJ-2019C038)。

摘　　要：In this wo rk,the phase-transitioned BSA(PTB)film using the mild and fast fabrication process adhered to the capillary inner wall uniformly,and the fabricated PTB film-coated capillary column was applied to realize open tubular capillary electrochromatography(OT-CEC)enantioseparation.The enantioseparation ability of PTB film-coated capillary was evaluated with eight pairs of chiral analytes including drugs and neurotransmitters,all achieving good resolution and symmetrical peak shape.For three consecutive runs,the relative standard deviations(RSD)of migration time for intra-day,inter-day,and column-tocolumn repeatability were in the range of 0.3%-3.5%,0.2%-4.9%and 2.1%-7.7%,respectively.Moreover,the PTB film-coated capillary column ran continuously over 300 times with high separation efficiency.Therefore,the coating method based on BSA self-assembly supramolecular film can be extended to the preparation of other proteinaceous capillary columns.

关 键 词：Open tubular capillary ELECTROCHROMATOGRAPHY Proteinaceous phase-transitioned film Bovine serum albumin(BSA) ENANTIOSEPARATION In-situ preparation 

分 类 号：O657.7[理学—分析化学]