Improvement in the risk assessment of oral leukoplakia through morphology-related copy number analysis  

作　　者：Xiaotian Li Lu Liu Jianyun Zhang Ming Ma Lisha Sun Xuefen Li Heyu Zhang Jianbin Wang Yanyi Huang Tiejun Li 

机构地区：[1]Department of Oral Pathology,Peking University School and Hospital of Stomatology&National Center of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental Materials,Beijing 100081,China [2]Beijing Advanced Innovation Center for Genomics(ICG),Biomedical Pioneering Innovation Center(BIOPIC),School of Life Sciences,Peking University,Beijing 100871,China [3]School of Life Sciences and Beijing Advanced Innovation Center for Structural Biology,Tsinghua University,Beijing 100084,China [4]Central Laboratory,Peking University School and Hospital of Stomatology,Beijing 100081,China [5]Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions,Chinese Academy of Medical Sciences(2019RU034),Beijing 100081,China [6]College of Chemistry and Molecular Engineering and Beijing National Laboratory for Molecular Sciences,Peking University,Beijing 100871,China [7]Institute for Cell Analysis,Shenzhen Bay Laboratory,Shenzhen 528107,China

出　　处：《Science China(Life Sciences)》2021年第9期1379-1391,共13页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(81671006,81300894,22050002,22050004);Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2019-I2M-5-038)。

摘　　要：Oral leukoplakia is the most common type of oral potentially malignant disorders and considered a precursor lesion to oral squamous cell carcinoma.However,a predictor of oral leukoplakia prognosis has not yet been identified.We investigated whether copy number alteration patterns may effectively predict the prognostic outcomes of oral leukoplakia using routinely processed paraffin sections.Comparison of copy number alteration patterns between oral leukoplakia with hyperplasia(HOL,n=22)and dysplasia(DOL,n=21)showed that oral leukoplakia with dysplasia had a higher copy number alteration rate(86%)than oral leukoplakia with hyperplasia(46%).Oral leukoplakia with dysplasia exhibited a wider range of genomic variations across all chromosomes compared with oral leukoplakia with hyperplasia.We also examined a retrospective cohort of 477 patients with oral leukoplakia with hyperplasia with detailed follow-up information.The malignant transformation(MT,n=19)and leukoplakia recurrence(LR,n=253)groups had higher frequencies of aneuploidy events and copy number loss rate than the free of disease(FD,n=205)group.Together,our results revealed the association between the degree of copy number alterations and the histological grade of oral leukoplakia and demonstrated that copy number alteration may be effective for prognosis prediction in oral leukoplakia patients with hyperplasia.

关 键 词：oral leukoplakia copy number alteration prognosis prediction 

分 类 号：R781[医药卫生—口腔医学]