Overexpression of anillin is related to poor prognosis in patients with hepatocellular carcinoma  

作　　者：Long-Hui Zhang Dong Wang Zhao Li Gang Wang Ding-Bao Chen Qian Cheng Shi-Hua Hu Ji-Ye Zhu 

机构地区：[1]Department of Hepatobiliary Surgery,Peking University People’s Hospital,Beijing 100044,China [2]Peking University Institute for Organ Transplantation,Beijing 100044,China [3]Beijing Key Laboratory of Liver Cirrhosis and Liver Cancer,Beijing 100044,China [4]Department of Pathology,Peking University People’s Hospital,Beijing 100044,China

出　　处：《Hepatobiliary & Pancreatic Diseases International》2021年第4期337-344,共8页国际肝胆胰疾病杂志（英文版）

基　　金：This study was supported by the grants from National Natural Science Foundation of China(81570590 and 81502509).

摘　　要：Background: Anillin(ANLN) is required for tumor growth. It has been proven that knockdown of ANLN effectively reduces the occurrence of hepatocellular carcinoma(HCC) in transgenic mice. However, the functional role of ANLN in HCC patients remains to be elucidated. Methods: Both microarray and TCGA project were used for the analyses of ANLN expression and regulation in HCC. The effect of ANLN on proliferation and cell cycle was detected by CCK-8, colony formation assay and flow cytometry. ANLN expression was measured by immunohistochemistry. Correlation between ANLN expression and clinicopathological features was assessed by Pearson Chi-square test and 5-year overall survival after liver resection was evaluated by Kaplan–Meier method. Results: Increased copy number, decreased methylation levels in the Cp G island and upregulated histone hypermethylation of ANLN were found in HCC. Knockdown of ANLN inhibited proliferation and induced G2/M phase arrest in SMMC-7721 cells. ANLN was mainly expressed in the nucleus and showed significantly higher expression levels in cancerous tissues than those in paired adjacent tissues. Moreover, nuclear ANLN expression levels in HCC metastases were significantly higher than those in primary HCC. The results of Cox proportional hazards regression model suggested that ANLN nuclear expression in HCC was an independent risk factor for poor 5-year overall survival of patients after liver resection. Conclusions: ANLN is a potential therapeutic target for HCC. Patients with nuclear ANLN overexpression in HCC tissue may need adjuvant therapy after liver resection.

关 键 词：ANILLIN DNA methylation Copy number variation Hepatocellular carcinoma PROGNOSIS 

分 类 号：R735.7[医药卫生—肿瘤]