子痫前期相关miR-23b-5p靶基因的预测及生物信息学分析  

Predication and Bioinformatics Analysis of Preeclampsia-Related miRNA-23b-5p Target Genes

作　　者：涂成城 陶峰[1] 储斌 陈红波[1] TU Cheng-cheng;TAO Feng;CHU Bin;CHEN Hong-bo(Department of Obstetrics and Gynecology,Maternal and Child Health Care Hospital Affiliated to Anhui Medical University,Hefei 230001,China)

机构地区：[1]安徽医科大学附属妇幼保健院妇产科,合肥230001

出　　处：《南昌大学学报（医学版）》2021年第4期1-6,共6页Journal of Nanchang University：Medical Sciences

基　　金：安徽省科技创新项目示范类项目(201707d08050003)。

摘　　要：目的预测并对子痫前期相关miR-23b-5p的候选靶基因进行生物信息学分析,为miR-23b-5p的机制研究及其靶基因的实验验证提供理论基础。方法在公共基因芯片数据库GEO(Gene Expression Omnibus)中选择子痫前期胎盘脐血miRNA差异表达的数据集GSE119799,选择表达显著下调的miR-23b-5p作为研究对象;使用在线分析网站RNA22-HAS、TargetScan及miRDB分别预测miR-23b-5p的靶基因,并利用韦恩图取其交集。使用富集分析网站DAVID对其交集靶基因进行GO功能富集分析、KEGG信号通路富集分析,并利用STRING网站对其进行蛋白质互作分析。结果获得的222个交集靶基因在生物过程上主要涉及干细胞分化的调控、胞质翻译负性调节等;在细胞组成上主要涉及膜外成分等;在分子功能上主要涉及翻译调节活性、金属离子结合等,主要参与MAPK信号通路、PI3K-AKT信号通路及钙信号通路等。蛋白互作分析显示TLR4是链接度最高的关键基因。结论miR-23b-5p可能通过影响滋养细胞侵袭、内皮细胞凋亡及炎症免疫反应等来参与子痫前期的发生发展,本研究为后续的靶基因验证及子痫前期发病机制的研究提供了理论基础和研究思路。Objective To predict and bioinformatically analyze preeclampsia-related miR-23b-5p target genes,and to provide a theoretical basis for the mechanisms of miR-23b-5p and the experimental verification of its target genes.Methods The data set GES119799 of miRNA differential expression in cord blood in preeclampsia was downloaded from Gene Expression Omnibus(GEO)database.The significantly down-regulated miR-23b-5p was selected as the study object.The target genes of miR-23b-5p were predicted using online databases miRDB,TargetScan and RNA22-HAS,and the intersection was drawn with Wayne map.The DAVID was used to perform GO function enrichment analysis and KEGG signal pathway enrichment analysis for its intersection target genes.The protein interaction analysis was conducted by STRING.Results The obtained 222 overlapping target genes were mainly involved in the regulation of stem cell differentiation and negative regulation of cytoplasmic translation in biological processes.In cell composition,they were mainly correlated with extramembrane components.In molecular function,they were mainly associated with translation regulation activity and metal ion binding.In addition,these genes were also related to MAPK,PI3K-Akt and calcium signaling pathways.Protein interaction analysis showed that TLR4 was the key gene with the highest degree of linkage.Conclusion miR-23b-5p may participate in the development of preeclampsia by affecting trophoblast invasion,endothelial cell apoptosis and inflammatory immune response.Our results provide theoretical basis and research ideas for the subsequent verification of target genes and the pathogenesis of preeclampsia.

关 键 词：子痫前期 MIRNA 靶基因 生物信息学 

分 类 号：R737.25[医药卫生—肿瘤] R34[医药卫生—临床医学]