Seed oil of Brucea javanica induces apoptosis through the PI3K/Akt signaling pathway in acute lymphocytic leukemia Jurkat cells  

作　　者：ZHANG Hong YIN Shi-Liang WANG Li-Hui JIA Li-Na SU Guang-Yue LIU Xiao-Qing ZHOU Fan BRESLIN Peter MENG Ran LI Qi-Yi YANG Jing-Yu WU Chun-Fu 

机构地区：[1]Department of Pharmacology,Shenyang Pharmaceutical University,Shenyang 110016,China [2]Department of Pharmacology,Shenyang Medical College,Shenyang 110034,China [3]Faculty of Functional Food And Wine,Shenyang Pharmaceutical University,Shenyang 110016,China [4]Department of Technology,Industry of Shenyang Pharmaceutical University of Lei Yun Shang Pharmaceutical Co.,Ltd.,Benxi 117004,China [5]Department of Hematology,General Hospital of Northern Theater Command,Shenyang 11016,China [6]Department of Molecular/Cellular Physiology,Loyola University Chicago,Chicago 60153,USA [7]School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Chinese Journal of Natural Medicines》2021年第8期608-620,共13页中国天然药物（英文版）

基　　金：supported by the Young Scientific and Technological Talents Seeding Program of Liaoning Education Office(No.2019LQN10);Shenyang Young and Middle-Aged Science and Technology Innovation Program(No.RC180308)。

摘　　要：Brucea javanica oil emulsion(BJOE)has been used to treat tumor in China for more than 40 years.However,its components and effectiveness in the treatment of acute lymphocytic leukemia(ALL)and its mechanism of anti-cancer activity remain unknown.In the current study,high-performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)was used to analyze the components of BJOE.Then,the anti-leukemia effects of BJOE were examined both in vitro and in vivo using ALL Jurkat cells and the p388 mouse leukemia transplant model,respectively.The primary ALL leukemia cells were also used to confirm the antileukemia effects of BJOE.The apoptotic-related results indicated that BJOE induced apoptosis in Jurkat cells and were suggestive of intrinsic apoptotic induction.Moreover,BJOE inhibited Akt(protein kinase B)activation and upregulated its downstream targets p53 and Fox O1(forkhead box gene,group O-1)to initiate apoptosis.The activation of GSK3βwas also involved.Our findings demonstrate that BJOE has anti-leukemia effects on ALL cells and can induce apoptosis in Jurkat cells through the phosphoinositide3-kinase(PI3 K)/Akt signaling pathway.

关 键 词：APOPTOSIS CANCER LEUKEMIA Brucea javanica oil emulsion 

分 类 号：R965[医药卫生—药理学]