灵芝菌种低温保藏保护剂配方研究  

Study on Protective Agent Formulation of Ganoderma lucidum Strain atLow Temperature Preservation

作　　者：王晓敏[1] 曾茜 魏善元[3] 陈旭[1] WANG Xiaomin;ZENG Qian;WEI Shanyuan;CHEN Xu(Institute of Soil and Fertilizer,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;School of Basic Medical,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Institute of Crop Germplasm Resources,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China)

机构地区：[1]贵州省农业科学院土壤肥料研究所,贵阳550006 [2]贵州中医药大学基础医学院,贵阳550025 [3]贵州省农业科学院农作物品种资源研究所,贵阳550006

出　　处：《耕作与栽培》2021年第4期46-49,共4页Tillage and Cultivation

基　　金：贵州省科技支撑计划项目(黔科合支撑[2019]2773号);贵州省科技重大专项(黔科合重大专项字[2019]3007号);贵州省科技支撑计划项目(黔科合支撑[2018]2781号)。

摘　　要：以二甲基亚砜、丙三醇、甲醇、蔗糖、聚乙二醇为灵芝菌种QL 02低温保藏的保护剂材料,对影响菌丝活性的关键保护剂种类进行筛选,通过响应面法对灵芝菌种QL 02号的低温保藏保护剂配方进行优化。结果表明,保护剂中甲醇含量、丙三醇含量和蔗糖含量为影响-20℃低温保藏灵芝菌株QL 02活性的关键因素;优化后的最优保护剂配方为蔗糖5.8%、甲醇8%、丙三醇4.7%。在此最优条件下,菌丝平均生长速度为5.13 mm·d^(-1)。In this study,dimethyl sulfoxide,glycerol,methanol,sucrose and polyethylene glycol were used as the protectant materials for low temperature preservation of Ganoderma lucidum strain QL 02.The key types of protectant affecting the activity of mycelia were screened,and then the formulation of the protectant for low temperature preservation of Ganoderma lucidum strain QL 02 was optimized by response surface methodology.The results showed that the contents of methanol,glycerol and sucrose in the protectant were the key factors affecting the activity of QL 02 preserved at low temperature.The optimal formula of protective agent after optimization was 5.8%sucrose,8%methanol and 4.7%glycerol.With the optimal conditions,the average growth rate of mycelia was 5.13 mm·d^(-1).

关 键 词：灵芝 低温保藏 保护剂 响应面法 

分 类 号：S567.31[农业科学—中草药栽培]