白菜型油菜开花时间的全基因组关联分析  

Genome-wide Association Analysis of Flowering Time in Brassica campestris

作　　者：江建霞 李延莉[1] 蒋美艳[1] 朱吉风 周熙荣[1] 王伟荣[1] 沈淳[2] 张俊英[1] 杨立勇[1] Jiang Jianxia;Li Yanli;Jiang Meiyan;Zhu Jifeng;Zhou Xirong;Wang Weirong;Shen Chun;Zhang Junying;Yang Liyong(Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201403;Qingpu Agrotechnology Extension Service Center,Shanghai,201700)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]上海市青浦区农业技术推广服务中心,上海201700

出　　处：《分子植物育种》2021年第16期5229-5240,共12页Molecular Plant Breeding

基　　金：上海市市级农口系统青年人才成长计划(沪农青字[2018]第1-5号)资助。

摘　　要：为定位白菜型油菜开花时间相关的候选基因,为白菜型油菜抽薹和开花时间遗传改良提供依据,以110份白菜型油菜组成的自然群体为研究材料,对它们进行花期调查和重测序分析。利用重测序获得的高质量的SNP集合对110份材料进行群体进化树分析、主成分分析和群体遗传结构分析和全基因组关联分析。花期观察结果表明,不同地理位置的白菜型油菜在开花时间上存在广泛差异。群体结构分析结果显示,110份材料可分为2个亚群,亚群内部分布较为集中,2个亚群之间的分布与白菜型油菜材料所处的地理位置密切相关。对开花时间进行全基因组关联分析,发现白菜型油菜全基因组衰减平均LD为19 kb,并获得了4个与开花时间表型显著关联的信号位点。对4个关联候选位点所在物理位置上下游一定区域内的相关基因进行功能注释,筛选得到白菜型油菜开花时间相关候选转录本9个,进一步分析发现9个转录本中一共包含4个候选基因,它们是拟南芥LOL1、CAT5和FAD8等的同源基因。本研究结果为获得白菜型油菜开花时间相关的候选基因,并利用候选基因进行花期调控和遗传改良提供一定理论基础和线索。In order to locate candidate genes related to flowering time, and to provide a basis for genetic improvement and flowering time of Brassica campestris, a natural population composed of 110 B. campestris was used as research materials. Flowering investigation and resequencing were performed on them. The high-quality SNP set obtained by resequencing was used for population evolutionary tree analysis, principal component analysis, population genetic structure analysis and a genome-wide association analysis. The observation results showed there were wide differences in flowering time between different types of B. campestris. The results of population structure analysis showed that 110 materials could be divided into two subgroups. The distribution within each phylogenetic tree was relatively concentrated, and the distribution between different subgroups was closely related to the geographical origin of the material. Genome-wide association analysis revealed that the average LD of the whole B. campestris attenuated LD was 19 kb, and 4 signal sites significantly associated with the flowering schedule type were obtained. Functional annotation was performed on related genes in a certain region upstream and downstream of the physical location of the four associated candidate sites, and 9 candidate transcripts related to flowering time were screened. Further analysis revealed that 9 transcripts contained a total of 4 candidate genes, which were homologous genes of Arabidopsis LOL1, CAT5 and FAD8. The results of this study could provide some theoretical basis and clues for obtaining candidate genes related to flowering time of B. campestris and using them to regulate flowering and genetic improvement.

关 键 词：白菜型油菜(Brassica campestris) 开花时间 连锁不平衡 全基因组关联分析 候选基因 

分 类 号：S565.4[农业科学—作物学]