不同含油量油菜籽代谢物差异分析  

Analysis on the Difference of Metabolites in Rapeseed with Different Oil Content

作　　者：常涛 李纲[2] 官梅[1] 张振乾[1] Chang Tao;Li Gang;Guan Mei;Zhang Zhenqian(Southern Grain and Oil Crop Collaborative Innovation Center,College of Agronomy,Hunan Agricultural University,Changsha,410128;Agricultural College,Yongzhou Vocational and Technical College,Yongzhou,425100)

机构地区：[1]湖南农业大学农学院,南方粮油作物协同创新中心,长沙410128 [2]永州职业技术学院农学院,永州425100

出　　处：《分子植物育种》2021年第16期5496-5502,共7页Molecular Plant Breeding

基　　金：国家重点基础研究发展计划(973计划)项目(2015CB150206)资助。

摘　　要：高含油油菜可提高单位面积产油量,因而受到广泛关注,但不同含油量油菜种子中代谢物的差异尚未清楚。本研究以2个含油量不同的甘蓝型油菜近等基因系授粉后20~35 d种子为材料,使用超高效液相色谱-四极杆飞行时间质谱分析不同含油量材料间代谢物差异。以低含油量材料为对照,共筛选出46种差异代谢物,其中24种与油脂合成通路相关,14种与脂肪酸合成通路相关,3种与糖代谢相关,2种与维生素代谢相关,1种与激素代谢相关,2种与花青素代谢相关。高含油油菜种子种固醇、花青素、硬脂酸、亚油酸等脂肪酸较高,亚麻酸含量较低。该结果有助于揭示油菜种子中油脂合成的机制,可为高含油油菜育种提供参考。High oil content rape can increase oil production per unit area because it was widely concerned. But the difference of metabolites in different oil content rape seeds was not clear. In this research, the metabolites of two near isogenic lines of Brassica napus with different oil content were analyzed by UPLC-Q-TOF/MS. The results showed that 46 kinds of differential metabolites were screened out, among which 24 were related to the pathway of oil synthesis, 14 to the pathway of fatty acid synthesis, 3 to the pathway of sugar metabolism, 2 to the pathway of vitamin metabolism, 1 to the pathway of hormone metabolism, and 2 to the pathway of anthocyanin metabolism.The fatty acids such as sterol, anthocyanin, stearic acid and linoleic acid were higher and linolenic acid was lower.The results were helpful to reveal the mechanism of oil synthesis in rape seeds and provide reference for high oil content rapeseed breeding.

关 键 词：油菜 代谢组学 含油量 超高效液相色谱 

分 类 号：S565.4[农业科学—作物学]