BMI-1和MMP-3基因多态性与汉族群体乳腺癌易感性的关联分析  

Association analysis of BMI-1 and MMP-3 gene polymorphisms with breast cancer susceptibility in Han nationality

作　　者：张明龙[1] 王秀华[2] 吕莹[2] 李鹏辉[3] 刘丹[1] 梅庆步[1] 刘万全 郑立红[1] ZHANG Ming-long;WANG Xiu-hua;LYU Ying;LI Peng-hui;LIU Dan;MEI Qing-bu;LIU Wan-quan;ZHENG Li-hong(Teaching and Research Section of Genetics,Qiqihar Medical University,Heilongjiang Province,Qiqihar 161006,China;Biotechnology Experimental Center,Qiqihar Medical University,Heilongjiang Province,Qiqihar 161006,China;College of Basic Medicine,Qiqihar Medical University,Heilongjiang Province,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院遗传学教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院生物技术实验中心,黑龙江齐齐哈尔161006 [3]齐齐哈尔医学院基础医学院,黑龙江齐齐哈尔161006

出　　处：《中国当代医药》2021年第26期14-17,共4页China Modern Medicine

基　　金：黑龙江省齐齐哈尔市科学技术计划一般指令性项目(SFGG-201909);齐齐哈尔医学科学院项目(QMSI2019Z-07)。

摘　　要：目的研究B细胞特异性白血病病毒插入位点-1(BMi-1)和基质金属蛋白酶-3(MMP-3)基因的3个单核苷酸多态性与汉族乳腺癌易感性的关联。方法基于病例-对照组研究方法,选取2014年1月至2018年7月齐齐哈尔医学院第二、三附属医院、齐齐哈尔建华医院和大庆油田总医院的524例汉族女性乳腺癌患者作为病例组,同期进行健康体检的518例健康体检者作为对照组,应用PCR-LDR技术对BMI-1基因rs1042059、rs201024480位点以及MMP-3基因rs3025058位点的基因多态性进行检测分型,用风险值(OR)和95%置信区间(CI)表示患病风险。结果病例组与对照组相比,MMP-3基因rs3025058位点等位基因频率和基因型分布在两组间有显著性差异(P=0.008);BMI-1基因rs201024480、rs1042059位点基因多态性在两组间比较,差异无统计学意义(P>0.05);校正混杂因素后,携带等位基因5A和基因型5A5A的群体乳腺癌发病风险分别提高1.51和1.48倍(OR=1.51,95%CI:1.17-1.94;P=0.001和OR=1.48,95%CI:1.16-1.91;P=0.001)。结论MMP-3基因rs3025058的多态性与乳腺癌发病风险存在关联,可作为乳腺癌易感基因的候选位点。Objective To study the association between three single nucleotide polymorphisms of B-cell-specific moloney leukemia virus insection site 1(BMI-1)and matrix metalloproteinase 3(MMP-3)genes and breast cancer susceptibility in Han nationality.Methods Using the case-control study method,from January 2014 to July 2018,a total of 524 Han women with breast cancer treated in the Second Affiliated Hospital of Qiqihar Medical College,the Third Affiliated Hospital of Qiqihar Medical College,Qiqihar Jianhua Hospital,and Daqing Oilfield General Hospital were selected as the case group,and 518 healthy people receiving healthy checkup in the same period were selected as the control group.PCR-LDR technology was used to detect and type the genetic polymorphisms of BMI-1 gene rs1042059,rs201024480 locus and MMP-3 gene rs3025058 locus,and the risk value(OR)and 95%confidence interval(CI)were used to express the risk of breast cancer.Results There were statistically significant differences in allele frequency and genotype distribution of MMP-3 gene rs3025058 locus between the case group and the control group(P=0.008).There was no statistically significant difference in gene polymorphism of BMI-1 gene rs1042059 locus and rs201024480 locus between the case group and the control group(P>0.05).After adjusting for confounding factors,the risk of breast cancer in the population carrying allele 5A and genotype 5A5A was increased by 1.51 and 1.48 times respectively(OR=1.51,95%CI:1.17-1.94;P=0.001 and OR=1.48,95%CI:1.16-1.91;P=0.001).Conclusion The polymorphism of MMP-3 gene rs3025058 is associated with the risk of breast cancer,and can be used as a candidate locus for breast cancer susceptibility genes.

关 键 词：乳腺癌 B细胞特异性白血病病毒插入位点-1 基质金属蛋白酶-3 基因多态性 易感性 

分 类 号：R737.9[医药卫生—肿瘤]