去卵巢小鼠股骨微结构的动态变化研究  

Dynamic changes of femur microarchitecture in ovariectomized mice

作　　者：邱宇 赵月阳 龙志敏 汪克建 徐凌[1] 贺桂琼 QIU Yu;ZHAO Yueyang;LONG Zhimin;WANG Kejian;XU Ling;HE Guiqiong(Department of Prosthodontics,Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences,Chongqing Key Laboratory of Oral Biomedical Engineering of Higher Education,Stomatological Hospital of Chongqing Medical University,Chongqing,401147;Department of Human Anatomy,Neuroscience Research Center,College of Basic Medical Sciences,Chongqing Medical University,Chongqing,400016,China)

机构地区：[1]重庆医科大学附属口腔医院修复科,口腔疾病与生物医学重庆市重点实验室,重庆市高校市级口腔生物医学工程重点实验室,重庆401147 [2]重庆医科大学基础医学院人体解剖学教研室,神经科学研究中心,重庆400016

出　　处：《第三军医大学学报》2021年第17期1627-1633,共7页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81671257);重庆高校创新团队建设计划资助项目(CXTDG201602006)。

摘　　要：目的探究去卵巢骨质疏松小鼠术后不同时期股骨微结构的动态变化。方法8周龄C57雌性小鼠分为去卵巢组(ovariectomy,OVX)和假手术组(sham ovariectomy,Sham),并根据术后不同时间将每组小鼠又分为1周、1个月及3个月组,分别代表绝经早、中、晚期。称质量后取小鼠股骨进行HE染色、免疫组织化学染色及Micro-CT扫描分析,评估各组股骨远端松质骨、股骨中段皮质骨微结构及成骨相关蛋白水平变化。结果小鼠松质骨形态参数:与Sham组相比,OVX组术后1周骨微结构未见明显破坏,术后1~3个月骨小梁断裂,骨髓腔增大,CT骨参数除Tb.Th无明显变化外(P>0.05),BV/TV,Tb.N和Tb.Sp均出现显著改变(P<0.01);Sham组中,直到术后3个月才出现较1周组骨量显著减少的表现(P<0.01);OVX各组中骨丢失情况随时间增加而加重(P<0.05,P<0.01)。Runx2表达水平与Micro-CT松质骨分析结果基本一致。皮质骨形态参数:各时段OVX组均未出现较Sham组的明显差异(P>0.05)。结论8周龄C57小鼠在OVX术后1周至1个月松质骨微结构明显受损,早于皮质骨变化,可作为骨质疏松病理机制和防治研究的重点阶段。Objective To explore the dynamic changes of femur microstructure in ovariectomized osteoporosis mice at different stages.Methods Female C57 mice(8 weeks old)were randomly divided into sham-operation(Sham)and ovariectomy(OVX)groups.Each group was subdivided into 1-week,1-month and 3-month subgroups on the basis of different postoperative time,which representeing early,middle and late menopause,respectively.The femurs were extracted for HE staining,immunohistochemistry and micro-CT analysis,and the changes in the bone microstructure of cancellous bone in the distal femurs,cortical bone in the middle femurs and the levels of osteogenic associated proteins in each group were evaluated.Results Compared with the Sham group,the bone microstructure of the OVX group showed no significant destruction at 1 week after operation,trabecula fracture and bone marrow cavity enlargement from 1 month to 3 months after operation,and significant changes in CT bone parameters BV/TV,Tb.N and Tb.Sp(P<0.01,P<0.001)except Tb.Th(P>0.05).In the Sham group,the bone mass was significantly reduced until 3 months after operation compared with that in the 1-week group(P<0.01).Bone loss in the OVX groups was worsened in a time dependent manner(P<0.05,P<0.01,P<0.001).The expression level of Runx2 by immunohistochemistry was consistent with the results of micro-CT analysis.No significant differences were found in morphologic indices of cortical bone between the Sham subgroups and the OVX subgroups(P>0.05).Conclusion Trabecular bone microarchitecture is impaired progressively after OVX operation in 8-week-old C57 mice from 1 week to 1 month,prior to cortical bone.This period can be regarded as the key time for studies on pathogenesis and prevention and treatment of osteoporosis.

关 键 词：骨质疏松 骨微结构 卵巢摘除术 动态变化 

分 类 号：R-332[医药卫生] R681.02