miRNA-30b调控ITGB3表达促进乳腺癌细胞凋亡  

MiRNA-30b promotes apoptosis in breast cancer cells by regulating ITGB3 gene expression

作　　者：钟科[1] 邓天芝[1] 黄大翠 Ke Zhong;Tianzhi Deng;Dacui Huang(Department of Thyroid and Breast Surgery,The First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院第一附属医院甲乳外科,成都市610500

出　　处：《中国肿瘤临床》2021年第15期761-766,共6页Chinese Journal of Clinical Oncology

基　　金：四川省卫生和计划生育委员会科研计划(编号:16PJ113);四川省教育厅创新团队与科研项目(编号:17ZB0136)资助。

摘　　要：目的:探讨微小RNA-30b(microRNA-30b,miRNA-30b)和整合素β3(integrinβ3,ITGB3)在乳腺癌组织中的表达及对乳腺癌细胞生物学行为的影响。方法:选择2016年3月至2019年3月于成都医学院第一附属医院收治的144例包括原位、浸润性和转移性乳腺癌患者的组织标本以及其癌旁组织,同时收集其临床病理资料。采用实时荧光定量PCR(RT-PCR)检测miRNA-30b和ITGB3的表达。构建miRNA-30b高表达的miRNA-30b模拟物和ITGB3高表达的miRNA-30b模拟物-pc DNA3.1-ITGB3,转染乳腺癌MCF-7细胞,同时将转染后的细胞皮下注射至小鼠体内。5-溴脱氧尿嘧啶核苷(BrdU)实验、流式细胞术、Transwell小室侵袭实验检测细胞增殖、凋亡、侵袭,Western blot检测细胞中的ITGB3表达。结果:RT-PCR结果显示,原位、浸润性和转移性乳腺癌组织中的miRNA-30b相对表达量分别为0.75、0.52和0.23,ITGB3 mRNA分别为2.17、3.76和5.43,与正常组织相比,差异均具有统计学意义(均P<0.001)。miRNA-30b高表达后,BrdU、流式细胞术、Transwell小室侵袭实验、Western blot检测结果显示,癌细胞的增殖、侵袭能力以及ITGB3表达明显降低,凋亡率升高(均P<0.001)。结论:在乳腺癌组织中miRNA-30b和ITGB3表达异常,miRNA-30b过表达能明显下调ITGB3表达,抑制乳腺癌细胞的增殖、侵袭,并促使其凋亡。Objective:To investigate microRNA-30b(miRNA-30b)and integrin β3(ITGB3)expression in breast cancer,and their effects on the biological behavior of breast cancer cells.Methods:One hundred and forty-four breast cancer tissue samples and their corresponding adjacent tissues,including in situ breast cancer and invasive and metastatic breast cancer tissues,were collected from the First Affiliated Hospital of Chengdu Medical College,from March 2016 to March 2019.Clinicopathological data were collected,and reverse transcription polymerase chain reaction(RT-PCR)was used to detect miRNA-30b and ITGB3 mRNA expression.Vectors for high miRNA-30b mimetic expression and high expression of ITGB3(miRNA-30b-mimetic-pc DNA3.1-ITGB3)were constructed and stably transfected into MCF-7 cells,which were subcutaneously injected into nude mice.A 5-bromine deoxyuridine nucleoside(BrdU)labeling experiment,flow cytometry,and Transwell chamber invasion experiments were used to assess the proliferation ability,apoptosis rate,and invasion ability of each stable cell line.Western blot was used to detect ITGB3 expression.Results:RT-PCR results indicated that the relative expression of miRNA-30b in the original tumor,infiltrating cells,and metastatic breast cancer tissue were 0.75,0.52,and 0.23,respectively,and ITGB3 mRNA levels were 2.17,3.76,and 5.43,respectively,compared to that in normal tissues.Observed differences were statistically significant(all P<0.001).BrdU,flow cytometry,Transwell chamber invasion experiments,and Western blot showed that in breast cancer cells,proliferation,invasion,and ITGB3 expression were significantly reduced,and the apoptosis rate was increased(all P<0.001).Conclusions:The expressions of miRNA-30b and ITGB3 are abnormal in breast cancer tissues.miRNA-30b overexpression can significantly downregulate ITGB3 expression,inhibit breast cancer cell proliferation and invasion,and promote their apoptosis.

关 键 词：微小RNA-30b 乳腺癌 整合素Β3 增殖 凋亡 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]