苦杏仁苷介导人肝癌细胞HuH-7凋亡的实验研究  

Experimental Study on Amygdalin Mediated Apoptosis of Human Hepatocellular Carcinoma Cell Line Huh-7

作　　者：王青瑛 王郁金 邢文文 闫颖 王相东 WANG Qing-ying;WANG Yu-jin;XING Wen-wen;YAN Ying;WANG Xiang-dong(Experimental Center of Traditional Chinese Medicine Diagnostics,School of Basic Medicine,Shaanxi University of Traditional Chinese Medicine,Xianyang,Shaanxi,712046,China;Teaching and Research Office of Traditional Chinese Medicine Diagnostics,School of Basic Medicine,Shaanxi University of Traditional Chinese Medicine,Xianyang,Shaanxi,712046,China)

机构地区：[1]陕西中医药大学基础医学院中医诊断学实验中心,陕西咸阳712046 [2]陕西中医药大学基础医学院中医诊断学教研室,陕西咸阳712046

出　　处：《现代生物医学进展》2021年第17期3227-3231,3216,共6页Progress in Modern Biomedicine

基　　金：陕西省中医管理局中医药科研项目(JCPT005)。

摘　　要：目的:通过体内体外实验探讨苦杏仁苷在肝癌中的抗肿瘤作用。方法:MTT法检测不同浓度的苦杏仁苷对肝癌HuH-7细胞存活率的影响;DAPI染色法观察苦杏仁苷介导HuH-7细胞的凋亡形态学变化;流式细胞术检测苦杏仁苷干预后HuH-7细胞凋亡率变化;Western Blot法检测细胞凋亡相关蛋白Bax、Bcl-2的表达,计算Bax/Bcl-2的比值。建立裸鼠HuH-7细胞移植瘤模型,观察苦杏仁苷对荷瘤裸鼠移植瘤体积的影响。结果:体外实验结果证实苦杏仁苷能够诱导人肝癌HuH-7细胞凋亡的发生(P<0.05)。随着苦杏仁苷浓度的增加,HuH-7细胞的存活率降低,凋亡率升高,干预后Bax/Bcl-2比值明显升高(P<0.05)。体内实验结果表明,裸鼠HuH-7细胞移植瘤的体积增长速度减慢(P<0.05)。结论:苦杏仁苷能够诱导人肝癌HuH-7细胞和裸鼠HuH-7细胞移植瘤细胞发生凋亡,减缓肿瘤生长,从而发挥抗肿瘤作用。Objective: To investigate the antitumor effect of Amygdalin in liver cancer through vitro and vivo experiments. Methods:MTT assay was used to detect different concentrations of amygdalin on the survival rate of HuH-7 cells;DAPI staining was used to observe the morphological changes of apoptosis induced by amygdalin in HuH-7 cells;flow cytometry was used to detect changes of apoptosis rate of HuH-7 cells after amygdalin intervention;expressions of apoptosis related proteins Bax and Bcl-2 were detected by Western blot, the ratio of Bax/Bcl-2 was calculated. Xenograft tumor model of HuH-7 cells in nude mice was established, and the effect of amygdalin on tumor volume in nude mice was observed. Results: The results of in vitro experiment confirmed that amygdalin could induce apoptosis of human hepatoma cell line HuH-7(P<0.05), with the concentration of amygdalin increased, the survival rate of HuH-7 cells decreased, the apoptosis rate increased, and the ratio of Bax/Bcl-2 increased significantly after intervention(P<0.05). The results of in vivo experiment showed that the growth rate of transplanted tumor of Huh-7 cells in nude mice slowed down(P<0.05). Conclusion:Amygdalin can induce apoptosis of HuH-7 cells and transplanted tumor cells of nude mice HuH-7 cells, slow down tumor growth, and play an anti-tumor effect.

关 键 词：苦杏仁苷 HUH-7细胞 凋亡 异体移植 

分 类 号：R-33[医药卫生] R735.7